Profiling of Serum Metabolites Using MALDI-TOF and Triple-TOF Mass Spectrometry to Develop a Screen for Ovarian Cancer
- 주제(키워드) Ovarian neoplasms , Low-mass ions , Serum metabolite , Hypoxanthine , Mass spectrometry
- 주제(기타) Oncology
- 설명문(일반) [Lee, Jun Hwa; Kim, Kyung-Hee; Woo, Sang Myung; Yoo, Byong Chul] Natl Canc Ctr, Res Inst, Biomarker Branch, 323 Ilsan Ro, Goyang 10408, South Korea; [Kim, Yun Hwan; Kim, Seung Cheol] Ewha Womans Univ, Sch Med, Dept Obstet & Gynecol, Div Gynecol Oncol,Mokdong Hosp, Seoul, South Korea; [Cho, Jae Youl] Sungkyunkwan Univ, Dept Genet Engn, Suwon, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- 발행기관 KOREAN CANCER ASSOCIATION
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000160304
- 본문언어 영어
- Published As http://dx.doi.org/10.4143/crt.2017.275
초록/요약
Purpose We sought to develop a matrix assisted laser desorption ionization-time of flight (MALDI-TOF)-based, ovarian cancer (OVC), low-mass-ion discriminant equation (LOME) and to evaluate a possible supportive role for triple-TOF mass analysis in identifying metabolic biomarkers. Materials and Methods A total of 114 serum samples from patients with OVC and benign ovarian tumors were subjected to MALDI-TOF analysis and a total of 137 serum samples from healthy female individuals and patients with OVC, colorectal cancer, hepatobiliary cancer, and pancreatic cancer were subjected to triple-TOF analysis. An OVC LOME was constructed by reference to the peak intensity ratios of discriminatory low-mass ion (LMI) pairs. Triple-TOF analysis was used to select and identify metabolic biomarkers for OVC screening. Results Three OVC LOMEs were finally constructed using discriminatory LMI pairs (137.1690 and 84.4119 m/z; 496.5022 and 709.7642 m/z; and 524.5614 and 709.7642 m/z); all afforded accuracies of > 90%. The LMIs at 496.5022 m/z and 524.5614 m/z were those of lysophosphatidylcholine (LPC) 16: 0 and LPC 18: 0. Triple-TOF analysis selected seven discriminative LMIs; each LMI had a specificity > 90%. Of the seven LMIs, four with a 137.0455 m/z ion at retention times of 2.04-3.14 minutes were upregulated in sera from OVC patients; the ion was identified as that derived from hypoxanthine. Conclusion MALDI-TOF-based OVC LOMEs combined with triple-TOF-based OVC metabolic biomarkers allow reliable OVC screening; the techniques are mutually complementary both quantitatively and qualitatively.
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