SPSB1 enhances ovarian cancer cell survival by destabilizing p21
- 주제(키워드) SPSB1 , Ovarian cancer , p21 destabilization , Ubiquitination , Cell survival
- 주제(기타) Biochemistry & Molecular Biology; Biophysics
- 설명문(일반) [Kim, Hyun-Jung; Kim, Hye Jin; Kim, Mi-Kyung; Bae, Moon Kyoung; Kim, Yun Hwan; Kim, Seung Cheol; Ju, Woong] Ewha Womans Univ, Dept Obstet & Gynecol, Coll Med, 25 Magokdong Ro 2 Gil, Seoul 07804, South Korea; [Sung, Hye Youn; Ahn, Jung-Hyuck] Ewha Womans Univ, Dept Biochem, Coll Med, Seoul, South Korea; [Kim, Hyun-Jung; Bae, Moon Kyoung; Sung, Hye Youn; Ahn, Jung-Hyuck; Kim, Yun Hwan; Kim, Seung Cheol; Ju, Woong] Ewha Womans Univ, Innovat Res Ctr Control & Prevent Womens Canc, Mokdong Hosp, Seoul, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 ACADEMIC PRESS INC ELSEVIER SCIENCE
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000160307
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.bbrc.2019.01.088
- PubMed https://pubmed.ncbi.nlm.nih.gov/30712944
초록/요약
SPRY domain-containing SOCS box protein 1 (SPSB1) is an E3 ligase adaptor protein with unknown functions in cancer cells. In this study, we found that SPSB1 knockdown markedly decreased the viability and migration of ovarian cancer cells, while ectopic SPSB1 overexpression in IL-3-dependent Ba/F3 cells significantly increased their proliferation rate compared with empty vector-transfected cells. SPSB1 knockdown significantly elevated p21 protein and mRNA levels and induced apoptosis in ovarian cancer cells, as evidenced by increased levels of cleaved PARP and decreased levels of Bcl-2. Notably, mechanistic investigations revealed that SPSB1 accelerated p21 destabilization by directly interacting with p21 and promoting its ubiquitin-mediated proteasomal degradation. Taken together, our findings provide novel insights into the role of SPSB1 in ovarian cancer cells. (C) 2019 Elsevier Inc. All rights reserved.
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