Enhanced production of poly-3-hydroxybutyrate (PHB) by expression of response regulator DR1558 in recombinant Escherichia coli
- 주제(키워드) dr1558 , PHB , Recombinant E. coli
- 주제(기타) Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science
- 설명문(일반) [Park, Sung-ho; Choi, Jong-il] Chonnam Natl Univ, Dept Biotechnol & Bioengn, Interdisciplinary Program Bioenergy & Biomat, Gwangju 61186, South Korea; [Kim, Gi Bae] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, SMESH Cross Generat Collaborat Lab, Metab & Biomol Engn Natl Res Lab,BK21 Plus Progra, Daejeon 34141, South Korea; [Kim, Hyun Uk] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn Plus Program, SMESH Cross Generat Collaborat Lab, Syst Biol & Med Lab,BK21 Plus Program, Daejeon 34141, South Korea; [Park, Si Jae] Ewha Womans Univ, Div Chem Engn & Mat Sci, Seoul 03760, South Korea
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCIENCE BV
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000160493
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.ijbiomac.2019.03.044
- PubMed https://pubmed.ncbi.nlm.nih.gov/30851327
초록/요약
During microbial production of target product, accumulation of by-products and target product itself may be toxic to host strain. Thus, development of abiotic stress tolerant strains are essential to achieve high productivity of target product with sustained metabolism. Expression of DR1558 from Deinococcus radiodurans, a response regulator in two-component signal transduction system, was reported to increase the tolerance against oxidative stress in Escherichia coli. In this study, the effect of overexpression of DR1558 was examined on poly-3-hydroxybutyrate (PHB) production in recombinant E coli expressing Ralstonia eutropha PHB biosynthesis genes. It was found that dr1558 overexpressing E. coli produced 531 g PHB/L and 9.24 g dry cell weight/L, while control strain produced 1.52 g PHB/L and 4.47 g dry cell weight/L in 48 h shake-flask cultivation. Transcriptional analysis of E coli suggested that DR1558 could improve the expression efficiency of the genes related to central carbon metabolism and threonine bypass pathway in PHB producing E. coli. When thrABC genes were overexpressed, PHB content was increased in recombinant E. coli, which suggests that stress-tolerant genes from extremophiles should be useful in the development of engineered strains for the production of bio-based products. (C) 2019 Elsevier B.V. All rights reserved.
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