Accelerated Development of Hippocampal Neurons and Limited Adhesion of Astrocytes on Negatively Charged Surfaces
- 주제(기타) Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, Multidisciplinary
- 설명문(일반) [Kim, Mi-Hee; Park, Ji Hun; Joo, Sunghoon; Park, Matthew; Choi, Ji Yu; Moon, Hye Won; Choi, Insung S.] Korea Adv Inst Sci & Technol, Dept Chem, Ctr Cell Encapsulat Res, Daejeon 34141, South Korea; [Hong, Daewha] Pusan Natl Univ, Dept Chem, Busan 46241, South Korea; [Hong, Daewha] Pusan Natl Univ, Chem Inst Funct Mat, Busan 46241, South Korea; [Kim, Yang-Gyun] Sungkyunkwan Univ, Dept Chem, Suwon 16419, Gyeonggi, South Korea; [Kang, Kyungtae] Kyung Hee Univ, Dept Appl Chem, Yongin 17104, Gyeonggi, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000160566
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acs.langmuir.7b03132
초록/요약
This work examines the development of primary neurons and astrocytes on thoroughly controlled functional groups. Negatively charged surfaces presenting carboxylate (COO-) or sulfonate (SO3-) groups prove beneficial to neuronal behavior, in spite of their supposed repulsive electrostatic interactions with cellular membranes. The adhesion and survival of primary hippocampal neurons on negatively charged surfaces are comparable to or slightly better than those on positively charged (poly-D-lysine-coated) surfaces, and neuritogenesis and neurite outgrowth are accelerated on COO- and SO3- surfaces. Moreover, such favorable influences of the negatively charged surfaces are only seen in neurons but not for astrocytes. Our results indicate that the in vitro developmental behavior of primary hippocampal neurons is sophisticatedly modulated by angstrom-sized differences in chemical structure or the charge density of the surface. We believe that this work provides new implications for understanding neuron-material interfaces as well as for establishing new ways to fabricate neuro-active surfaces.
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