Lamb wave-based molecular diagnosis using DNA hydrogel formation by rolling circle amplification (RCA) process
- 주제(키워드) Acoustic streaming , DNA hydrogel , Gene amplification , Lamb wave , Molecular diagnosis
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier Ltd
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000160700
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.bios.2019.111496
- PubMed https://pubmed.ncbi.nlm.nih.gov/31302395
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Recent developments in microfluidics enable the lab-on-a-chip-based molecular diagnosis. Rapid and accurate diagnosis of infectious diseases is critical for preventing the transmission of the disease. Here, we characterize a Lamb wave-based device using various parameters including the contact angle and viscosity of the sample droplet, the applied voltage, and the temperature increase. Additionally, we demonstrate the functionality of the Lamb wave-based device in clinical application. Optimal temperature for rolling circle amplification (RCA) process is 30 °C, and it was achieved by Lamb wave generation at 17 V. Gene amplification due to RCA process could be detected by viscosity increase due to DNA hydrogel formation in a sample droplet, which induced the acoustic streaming velocity of suspended particles to be decreased. In our Lamb wave-based device, isothermal amplification of target nucleic acids could be successfully detected within 30 min using 10 μL of sessile droplet, and was validated by comparing that of commercial real-time fluorescence analysis. Our device enables simple and low-cost molecular diagnosis, which can be applied to resource-limited clinical settings. © 2019 Elsevier B.V.
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