Endocytosing Escherichia coli as a Whole-Cell Biocatalyst of Fatty Acids
- 주제(키워드) biotransformation , caveolin-1 , endocytosis , fatty acid , heterologous caveolae
- 등재 SCIE, SCOPUS
- 발행기관 American Chemical Society
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000160726
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acssynbio.8b00519
- PubMed https://pubmed.ncbi.nlm.nih.gov/31018087
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Whole cell biocatalysts can be used to convert fatty acids into various value-added products. However, fatty acid transport across cellular membranes into the cytosol of microbial cells limits substrate availability and impairs membrane integrity, which in turn decreases cell viability and bioconversion activity. Because these problems are associated with the mechanism of fatty acid transport through membranes, a whole-cell biocatalyst that can form caveolae-like structures was generated to promote substrate endocytosis. Caveolin-1 (CAV1) expression in Escherichia coli increased both the fatty acid transport rate and intracellular fatty acid concentrations via endocytosis of the supplemented substrate. Furthermore, fatty-acid endocytosis alleviated substrate cytotoxicity in E. coli. These traits attributed to bacterial endocytosis resulted in dramatically elevated biotransformation efficiencies in fed-batch and cell-recycle reaction systems when caveolae-forming E. coli was used for the bioconversion of ricinoleic acid (12-hydroxyoctadec-9-enoic acid) to (Z)-11-(heptanoyloxy) undec-9-enoic acid. We propose that CAV1-mediated endocytosing E. coli represents a versatile tool for the biotransformation of hydrophobic substrates. © 2019 American Chemical Society.
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