Efficient production of gamma-aminobutyric acid using Escherichia coli by co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter
- 주제(키워드) Co-localization , GABA , Protein scaffold , Synthetic biology
- 주제(기타) Biotechnology & Applied Microbiology
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 SPRINGER HEIDELBERG
- 발행년도 2016
- URI http://www.dcollection.net/handler/ewha/000000161991
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s10295-015-1712-8
초록/요약
Gamma-aminobutyric acid (GABA) is an important bio-product, which is used in pharmaceutical formulations, nutritional supplements, and biopolymer monomer. The traditional GABA process involves the decarboxylation of glutamate. However, the direct production of GABA from glucose is a more efficient process. To construct the recombinant strains of Escherichia coli, a novel synthetic scaffold was introduced. By carrying out the co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter, we redirected the TCA cycle flux to GABA pathway. The genetically engineered E. coli strain produced 1.08 g/L of GABA from 10 g/L of initial glucose. Thus, with the introduction of a synthetic scaffold, we increased GABA production by 2.2-fold. The final GABA concentration was increased by 21.8 % by inactivating competing pathways.
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