Engineering the intracellular metabolism of Escherichia coli to produce gamma-aminobutyric acid by co-localization of GABA shunt enzymes
- 주제(키워드) Gamma-aminobutyric acid , Gamma-aminobutyric acid shunt , Co-localization , Escherichia coli , Gamma-Scaffold system
- 주제(기타) Biotechnology & Applied Microbiology
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 SPRINGER
- 발행년도 2016
- URI http://www.dcollection.net/handler/ewha/000000162005
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s10529-015-1982-2
초록/요약
To direct the carbon flux from Krebs cycle into the gamma-aminobutyric acid (GABA) shunt pathway for the production of GABA by protein scaffold introduction in Escherichia coli. Escherichia coli was engineered to produce GABA from glucose by the co-localization of enzymes succinate semialdehyde dehydrogenase (GadD), GABA aminotransferase (PuuE) and GABA transporter (GadC) by protein scaffold. 0.7 g GABA l(-1) was produced from 10 g glucose l(-1) while no GABA was produced in wild type E. coli. pH 6 and 30 A degrees C were optimum for GABA production, and GABA concentration increased to 1.12 g GABA l(-1) when 20 g glucose l(-1) was used. When competing metabolic networks were inactivated, GABA increased by 24 % (0.87 g GABA l(-1)). The novel GABA production system was constructed by co-localization of GABA shunt enzymes.
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