Isolation and Proteomic Analysis of a Chlamydomonas reinhardtii Mutant with Enhanced Lipid Production by the Gamma Irradiation Method
- 주제(키워드) Chlamydomonas reinhardtii , lipid content , mutant , proteome
- 주제(기타) Biotechnology & Applied Microbiology
- 주제(기타) Microbiology
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS, KCI등재
- 발행기관 KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- 발행년도 2016
- URI http://www.dcollection.net/handler/ewha/000000162094
- 본문언어 영어
- Published As http://dx.doi.org/10.4014/jmb.1605.05057
초록/요약
In this study, an enhanced lipid-producing mutant strain of the microalga Chlamydomonas reinhardtii was developed by gamma irradiation. To induce the mutation, C. reinhardtii was gamma irradiated at a dose of 400 Gy. After irradiation, the surviving cells were stained with Nile red. The mutant (Cr-4013) accumulating 20% more lipid than the wild type was selected. Thin-layer chromatography revealed the triglyceride and free fatty acid contents to be markedly increased in Cr-4013. The major fatty acids identified were palmitic acid, oleic acid, linoleic acid, and linolenic acid. Random amplified polymeric DNA analysis showed partial genetic modifications in Cr-4013. To ascertain the changes of protein expression in the mutant strain, two-dimensional electrophoresis was conducted. These results showed that gamma radiation could be used for the development of efficient microalgal strains for lipid production.
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