Microbial compositional changes in broiler chicken cecal contents from birds challenged with different Salmonella vaccine candidate strains
- 주제(키워드) Salmonella , Vaccine , Poultry , Microbiome , Sequencing
- 주제(기타) Immunology
- 주제(기타) Medicine, Research & Experimental
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCI LTD
- 발행년도 2017
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000162130
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.vaccine.2017.04.073
초록/요약
Previously, we constructed and characterized the vaccine efficacy of Salmonella Typhimurium mutant strains in poultry with either inducible mviN expression (P-BAD-mviN) or methionine auxotrophy (Delta Delta metRmetD). The aim of the present study was to assess potential impact of these Salmonella vaccine strains on the cecal microbiota using a next generation sequencing (NGS). The cecal microbial community obtained from unvaccinated (group 1) and vaccinated chickens (group 2, vaccinated with PBAD-mviN; group 3, vaccinated with wild type; group 4, vaccinated with Delta Delta metRmetD) were subjected to micro-biome sequencing analysis with an Illumina MiSeq platform. The NGS microbiome analysis of chicken ceca revealed considerable changes in microbial composition in the presence of the different vaccine strains and exhibited detectable patterns of distinctive clustering among the respective groups (the R value of unweighted PCoA plot was 0.68). The present study indicates that different S. Typhimurium vaccine strains can differentially influence the microbiota of the ceca in terms of presence but not in the relative abundance of microbiota. (C) 2017 Elsevier Ltd. All rights reserved.
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