Control strategy of displacement processes to sense biothiols via fluorescent changes
- 주제(키워드) Biothiol selective probes , Cu2+ selective fluorescent probes , Displacement approach , Fluorescent imaging probes , Fluorescent probe for GSH
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier Ltd
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000162305
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.dyepig.2019.107871
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Two different bis-pyrene derivatives BP-1 and BP-2 were synthesized, which showed efficient fluorescent quenching by Cu2+. The addition of biothiols, such as GSH, Cys, and Hcy, restored their fluorescent emissions. Differences in the affinities of BP-1 and BP-2 for Cu2+ were successfully applied to the different detection limits and the linear detection range of biothiols. An NH moiety and a sulfur atom in the linker were the only differences between BP-1 and BP-2. Since BP-1 bound Cu2+ more tightly than BP-2, BP-1-Cu2+ required more GSH, Cys, and Hcy equivalents to restore the fluorescent emission. In contrast, only two equivalents of biothiols restored the original emission of BP-2-Cu2+. As a proof-of-concept, we demonstrated that molecular recognition chemistry and host-gest chemistry, more specifically the modulation of metal ion recognition, can be successfully applied to biothiol detection, especially over different concentration ranges. BP-1 showed a fluorescent quenching effect in living cells upon the addition of Cu2+ and BP-1-Cu2+ was also successfully applied to imaging biothiols in living cells. © 2019
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