Microbial production of O-methylated flavanones from methylated phenylpropanoic acids in engineered Escherichia coli
- 주제(키워드) Escherichia coli , Homoeriodictyol , Methylated flavanone , Microbial production , Phenylpropanoic acid
- 등재 SCIE, SCOPUS
- 발행기관 Springer Verlag
- 발행년도 2019
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000162387
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s10295-019-02239-6
- PubMed https://pubmed.ncbi.nlm.nih.gov/31595455
초록/요약
Methylated flavonoids possess improved bioactivities compared to their unmethylated counterparts. In this study, for the efficient production of O-methylated flavonoids from simple methylated phenylpropanoic acids, a recombinant Escherichia coli strain expressing 4-coumarate:coenzyme A ligase (4CL) from Oryza sativa and chalcone synthase (CHS) from Hordeum vulgare was constructed; this strain produced significant amount of homoeriodictyol (~ 52 mg/L) as well as a few amount of hesperetin (0.4 mg/L), respectively, from ferulic acid and 4-methylcaffeic acid. This demonstrates, for the first time, that the scarce but valuable methylated flavanones can be successfully produced from methylated phenylpropanoic acids in a microbial host via an artificial biosynthetic pathway consisting of 4CL and CHS that can accept O-methylated precursors. © 2019, Society for Industrial Microbiology and Biotechnology.
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