Over-expression of p190RhoGEF enhances B-cell activation and germinal center formation in T-cell-dependent humoral immune responses
- 주제(키워드) B cells , CD40 signaling , humoral immune responses , p190RhoGEF , plasma cells
- 등재 SCIE, SCOPUS
- 발행기관 John Wiley and Sons Inc.
- 발행년도 2019
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000162421
- 본문언어 영어
- Published As http://dx.doi.org/10.1111/imcb.12286
- PubMed https://pubmed.ncbi.nlm.nih.gov/31361349
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Previously, we reported induced expression of the p190 Rho guanine nucleotide exchange factor (p190RhoGEF, ARHGEF28) following CD40 stimulation of B cells isolated from mouse spleen. We also reported that p190RhoGEF and a downstream effector molecule RhoA are required for B-cell differentiation, especially for the induction of the plasma cell (PC) differentiation. This study investigates the role of p190RhoGEF in B-cell biology in vivo, using p190RhoGEF transgenic (TG) mice that overexpress a wild-type full gene in B cells. Immunization of these mice with T-cell-dependent antigen showed that populations of germinal center B cells and PCs were significantly increased in TG mice. Furthermore, similar results were shown in recombination activating 1 (Rag1) knockout mice that were reconstituted with B cells isolated from TG mice in combination with T cells isolated from littermate control mice. Analyses of isotype class switching and transcription factors involved in a germinal center reaction and PC differentiation also supported the findings from the cellular responses. These results suggest that p190RhoGEF may play a role in the stage of PC differentiation during T-cell-dependent humoral immune responses. © 2019 Australian and New Zealand Society for Immunology Inc.
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