A 3D-printed polycaprolactone/beta-tricalcium phosphate mandibular prosthesis: A pilot animal study
- 주제(키워드) Osteogenesis , Stem cell , 3D printing , Graft material
- 주제(기타) Medicine, Research & Experimental; Otorhinolaryngology
- 설명문(일반) [Park, Jae Hong; Lee, Chi-Kyou; Ban, Myung Jin] Soonchunhyang Univ, Dept Otorhinolaryngol Head & Neck Surg, Coll Med, Asan, South Korea; [Jang, Si-Hyong] Soonchunhyang Univ, Dept Pathol, Coll Med, Asan, South Korea; [Park, Hae Sang] Hallym Univ, Coll Med, Chuncheon Sacred Heart Hosp, Dept Otorhinolaryngol Head & Neck Surg, Chunchon, South Korea; [Jung, Soo Yeon; Oh, Hyun Ju] Ewha Womans Univ, Coll Med, Dept Otorhinolaryngol Head & Neck Surg, Anyangchun Ro 1071, Seoul 07985, South Korea; [Kim, Ha Yeong] Ewha Womans Univ, Coll Med, Dept Mol Med, Seoul, South Korea; [Kim, Byeong Kook] Ajou Univ, Med Ctr, Cell Therapy Ctr, Suwon, South Korea; [Lee, Sang Jin; Kim, Han Su] Wake Forest Sch Med, Wake Forest Inst Regenerat Med, Winston Salem, NC 27101 USA
- 등재 SCIE, SCOPUS
- 발행기관 WILEY
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000165802
- 본문언어 영어
- Published As http://dx.doi.org/10.1002/lary.27908
- PubMed https://pubmed.ncbi.nlm.nih.gov/30861134
초록/요약
Objective In this study, we assessed the effectiveness of a tonsil-derived mesenchymal stem cell (TMSC)-transplanted polycaprolactone/beta-tricalcium phosphate prosthesis (specifically designed for easier fixing and grafting with a single scaffold) on rabbit mandible osteogenesis. Methods The mandibles of 18 rabbits were exposed, and 10 x 8-mm bone defects were made. Two rabbits did not receive implants; four were reconstructed with the scaffold control (SC) (SC group); four were reconstructed with scaffolds soaked in peripheral blood (PB) (PB group); four were reconstructed with TMSC-transplanted scaffolds (TMSC group); and four were reconstructed with differentiated osteocyte-transplanted scaffolds (DOC) (DOC group). Each rabbit was sacrificed 12 weeks after surgery, and the area of new bone formation was investigated by mechanical testing, histology, and micro-computed tomography. Results More extended and denser new bone masses were observed in the TMSC and DOC groups, although fibrosis and vascular formation levels were similar in all groups, suggesting that the dual-structured scaffold alone provides a good environment for bone attachment and regeneration. The bone volumes of representative scaffolds from the SC, PB, TMSC, and DOC groups were 43.12, 48.35, 53.10, and 57.44% of the total volumes, respectively. Conclusion The design of the scaffold resulted in effective osteogenesis, and TMSCs showed osteogenic potency, indicating that their combination could enable effective bone regeneration. Level of Evidence NA Laryngoscope, 130:358-366, 2020
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