Cofactor specificity engineering of a long-chain secondary alcohol dehydrogenase from Micrococcus luteus for redox-neutral biotransformation of fatty acids
- 주제(기타) Chemistry, Multidisciplinary
- 설명문(일반) [Seo, Eun-Ji; Kim, Myeong-Ju; Park, Jin-Byung] Ewha Womans Univ, Dept Food Sci & Engn, Seoul 03760, South Korea; [Kim, Hye-Ji; Kim, Jeong-Sun] Chonnam Natl Univ, Dept Chem, Gwangju 61186, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 ROYAL SOC CHEMISTRY
- 발행년도 2019
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000165871
- 본문언어 영어
- Published As http://dx.doi.org/10.1039/c9cc06447h
- PubMed https://pubmed.ncbi.nlm.nih.gov/31728457
초록/요약
Structure-based engineering of a NAD(+)-dependent secondary alcohol dehydrogenase from Micrococcus luteus led to a 1800-fold increase in catalytic efficiency for NADP(+). Furthermore, the engineered enzymes (e.g., D37S/A38R/V39S/T15I) were successfully coupled to a NADPH-dependent Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440 for redox-neutral biotransformations of C18 fatty acids into C9 chemicals.
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