Detection of nonylphenol with a gold-nanoparticle-based small-molecule sensing system using an ssDNA aptamer
- 주제(키워드) Aptamer , Detergent , Endocrine disruptors , Nonylphenol , Reduced graphene oxide , SELEX
- 등재 SCIE, SCOPUS
- OA유형 Green Published, gold
- 발행기관 MDPI AG
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000165896
- 본문언어 영어
- Published As http://dx.doi.org/10.3390/ijms21010208
- PubMed https://pubmed.ncbi.nlm.nih.gov/31892242
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Endocrine-disrupting chemicals (EDCs) threaten many kinds of life throughout the world. These compounds function the same as sexual hormones, inducing precocious puberty, gynecomastia, etc., in the human body. To prevent excess exposure to nonylphenol (NP), a simple and rapid detection system is needed. In this study, we develop a nonylphenol-specific aptamer from a random single-stranded DNA library and test a rapid sensor system based on the aptamer and gold nanoparticles (AuNPs). The aptamer was screened by a methodology involving reduced graphene oxide (rGO). As a result of screening and sequencing, a DNA aptamer was developed that recognizes the target with high binding affinity (Kd = 194.2 ± 65.9 nM) and specificity. The sensor system developed using the aptamer and gold nanoparticles is sensitive (LOD = 2.239 nM). Circular dichroism (CD) spectrometry results show that the free aptamer binds to the target molecule. The aptamer was characterized using gold nanoparticles to measure UV absorbance. Our results suggest that the sensor system developed using this aptamer is useful for field diagnosis of small molecules. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
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