Development of Metabolically Engineered Corynebacterium glutamicum for Enhanced Production of Cadaverine and Its Use for the Synthesis of Bio-Polyamide 510
- 주제(키워드) Corynebacterium glutamicum , cadaverine , Hafnia alvei lysine decarboxylase , PAS10
- 주제(기타) Chemistry, Multidisciplinary; Green & Sustainable Science & Technology; Engineering, Chemical
- 설명문(일반) [Kim, Hee Taek; Hyun, Sung Mm; Khang, Tae Uk; Sohn, Yu Jung; Song, Bong Keun; Joo, Jeong Chan] Korea Res Inst Chem Technol, Adv Convergent Chem Div, Biobased Chem Ctr, POB 107,141 Gajeong Ro, Daejeon 34114, South Korea; [Baritugo, Kei-Anne; Sohn, Yu Jung; Park, Si Jae] Ewha Womans Univ, Div Chem Engn & Mat Sci, 52 Ewhayeodae Gil, Seoul 03760, South Korea; [Hyun, Sung Mm; Park, Kyungmoon] Hongik Univ, Dept Biol & Chem Engn, 2639 Sejong Ro, Sejong Si 30016, South Korea; [Khang, Tae Uk] Korea Univ, Dept Chem & Biol Engn, 145 Anam Ro, Seoul 02841, South Korea; [Kim, Il-Kwon] DAESANG Corp, Bioproc R&D Ctr, Icheon Si 17384, Gyeonggi Do, South Korea; [Hwang, Yong Taek] Lotte Chem, 115 Gajeongbuk Ro, Daejeon 34110, South Korea; [Lee, Sang Yup] Inst Sci & Technol KAIST, Metabol & Biomol Engn Natl Res Lab, SMESH Cross Generat Collaborat Lab,Plus Program B, Inst BioCentury Korea Adv,Dept Chem & Biomol Engn, Daejeon 34141, South Korea
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2020
- URI http://www.dcollection.net/handler/ewha/000000165901
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acssuschemeng.9b04693
초록/요약
Lysine decarboxylases (LDCs) from Escherichia coli, Lactobacillus saerimneri, Streptomyces coelicolor, Selemonas ruminantium, Hafnia alvei, and Vibrio vulnificus were examined for their ability to enhance the fermentative production of cadaverine in Corynebacterium glutamcium. Among these LDCs, the plasmid-based expression of the H. alvei LDC gene (ldcC(Ha)) under strong promoters (P-H30, P-H36) produced high concentrations of cadaverine (11.411.5 g/L), which is similar to 12.5 g/L of cadaverine produced by the plasmid-based expression of the E. coli LDC gene (ldcC(Ec)) by the PH30 promoter in our previous report. The production of cadaverine in batch (30.8 g/L) and fed-batch (93.7 g/L) fermentation cultures using recombinant strain Corynebacterium glutamicum H30HaLDC expressing plasmid-borne ldcC(Ha) under control of the PH30 promoter was 18 and 14% higher than that obtained using C. glutamicum P-H30 expressing plasmid-borne ldcC(Ec) under control of the PH30 promoter, in which production was only 26 and 82.2 g/L in batch and fed-batch cultures, respectively. Finally, C. glutamicum GH30HaLDC was constructed by integration of ldcC(Ha) into C. glutamicum PKC at the lysE site. C. glutamicum GH30HaLDC produced 125 g/L of cadaverine in fed-batch culture, which was 20% higher than the cadaverine production of 104 g/L by C. glutamicum G-H30 in our previous report. Cadaverine produced by fed-batch culture of C. glutamicum GH30HaLDC was purified and used for the synthesis of bio-based polyamide PA510 by copolymerization with sebacic acid. Synthesized PA510 showed thermal and material properties comparable to those of chemical-based PA510.
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