Two-Photon Fluorescence Probe for Selective Monitoring of Superoxide in Live Cells and Tissues
- 주제(기타) Chemistry, Analytical
- 설명문(일반) [Chen, Liyan; Wu, Di; Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea; [Cho, Myoung Ki; Hwan Myung Kim] Ajou Univ, Dept Chem & Energy Syst Res, Suwon 16499, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2019
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000166121
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acs.analchem.9b03937
- PubMed https://pubmed.ncbi.nlm.nih.gov/31631657
초록/요약
The abnormal location or generation of superoxide radical anion (O-2(center dot-)) are implicated in many diseases, including cancers; thus, development of an efficient method to detect O(2)(center dot- )is of great importance. Inspired by the fluorophore-governed selective manner to O-2(center dot-) and peroxynitrite (ONOO-) of previously reported phosphinate-based fluorescence probes, in this contribution, a phosphinothioate-containing probe, TPP, was designed. The probe exhibited easy accessibility through a one-step sequence and good photostability and biocompatibility. Interestingly, TPP showed high specificity and sensitivity to O-2(center dot-) over other reactive oxygen species/nitrogen species including ONOO. Furthermore, with the assistance of two-photon microscopy, TPP was successfully applied for imaging endogenous O-2(center dot-) in live cells and tissues.
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