Plasma proteomic profiling of young and old mice reveals cadherin-13 prevents age-related bone loss
- 주제(키워드) aging , bone , plasma proteins , proteomics , osteoclast differentiation
- 주제(기타) Cell Biology
- 주제(기타) Geriatrics & Gerontology
- 설명문(일반) [Yang, Yong Ryoul; Kang, Jae Sook; Shin, Yeo Jin; Lee, Seung Min; Lee, Jaemin; Lee, Kwang-Pyo; Kwon, Ki-Sun] Korea Res Inst Biosci & Biotechnol, Aging Res Ctr, Daejeon, South Korea; [Kabir, Mohammad Humayun; Ju, Shinyeong; Lee, Cheolju] Korea Inst Sci & Technol, Ctr Theragnosis, Seoul, South Korea; [Park, Jin Hee; Lee, Soo Young] Ewha Womans Univ, Dept Life Sci, Res Ctr Cellular Homeostasis, Seoul, South Korea; [Park, Jae-Il] Chonnam Natl Univ, Gwangju Ctr, Korea Basic Sci Inst, Gwangju, South Korea; [Kang, Jae Sook; Kwon, Ki-Sun] Korea Univ Sci & Technol UST, KRIBB Sch Biosci, Dept Funct Genom, Daejeon, South Korea; [Ju, Shinyeong] Hanyang Univ, Dept Life Sci, Seoul, South Korea; [Ju, Shinyeong] Hanyang Univ, Res Inst Nat Sci, Seoul, South Korea; [Kim, Seokho] Dong A Univ, Coll Hlth Sci, Dept Med Biotechnol, Busan, South Korea; [Lee, Cheolju] Kyung Hee Univ, KHU KIST Dept Converging Sci & Technol, Seoul, South Korea; [Lee, Cheolju] Korea Univ Sci & Technol, KIST Sch, Div Biomed Sci & Technol, Seoul, South Korea; [Kabir, Mohammad Humayun] Incepta Vaccine Ltd, Dhamrai, Kalampur Dhaka, Bangladesh
- 등재 SCIE, SCOPUS
- OA유형 Green Published, gold
- 발행기관 IMPACT JOURNALS LLC
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000168758
- 본문언어 영어
- Published As https://dx.doi.org/10.18632/aging.103184
- PubMed https://pubmed.ncbi.nlm.nih.gov/32396872
초록/요약
The blood exhibits a dynamic flux of proteins that are secreted by the tissues and cells of the body. To identify novel aging-related circulating proteins, we compared the plasma proteomic profiles of young and old mice using tandem mass spectrometry. The expression of 134 proteins differed between young and old mice. We selected seven proteins that were expressed at higher levels in young mice, and confirmed their plasma expression in immunoassays. The plasma levels of anthrax toxin receptor 2 (ANTXR2), cadherin-13 (CDH-13), scavenger receptor cysteine-rich type 1 protein M130 (CD163), cartilage oligomeric matrix protein (COMP), Dickkopf-related protein 3 (DKK3), periostin, and secretogranin-1 were all confirmed to decrease with age. We then investigated whether any of the secreted proteins influenced bone metabolism and found that CDH-13 inhibited osteoclast differentiation. CDH 13 treatment suppressed the receptor activator of NF-kappa B ligand (RANKL) signaling pathway in bone marrow-derived macrophages, and intraperitoneal administration of CDH-13 delayed age-related bone loss in the femurs of aged mice. These findings suggest that low plasma CDH-13 expression in aged mice promotes aging-associated osteopenia by facilitating excessive osteoclast formation. Thus, CDH-13 could have therapeutic potential as a protein drug for the prevention of osteopenia.
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