Characterization of human cardiac mesenchymal stromal cells and their extracellular vesicles comparing with human bone marrow derived mesenchymal stem cells
- 주제(키워드) Cardiovascular disease , Extracellular vesicles , Mesenchymal stem cell , Regeneration
- 주제(기타) Biochemistry & Molecular Biology
- 설명문(일반) [Kang, In Sook; Jang, Yangsoo] Yonsei Univ, Grad Sch, Coll Med, Seoul 03722, South Korea; [Kang, In Sook] Ewha Womans Univ, Mokdong Hosp, Sch Med, Dept Internal Med, Seoul 07804, South Korea; [Suh, Joowon; Lee, Mi-Ni; Lee, Chaeyoung; Jin, Jing; Oh, Goo Taeg] Ewha Womans Univ, Dept Life Sci, Seoul 03760, South Korea; [Suh, Joowon; Lee, Mi-Ni; Lee, Chaeyoung; Jin, Jing; Oh, Goo Taeg] Ewha Womans Univ, Coll Nat Sci, Seoul 03760, South Korea; [Lee, Chaeyoung] Univ Toronto, Fac Arts & Sci, Toronto, ON M5S 1A1, Canada; [Lee, Changjin] Rosetta Exosome Inc, Seoul 06159, South Korea; [Yang, Young Il] Inje Univ, Paik Inst Clin Res, Coll Med, Busan 47392, South Korea; [Jang, Yangsoo] Yonsei Univ, Severance Cardiovasc Hosp, Div Cardiol, Coll Med, Seoul 03722, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- OA유형 Green Published, gold
- 발행기관 KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000168763
- 본문언어 영어
- Published As https://dx.doi.org/10.5483/BMBRep.2020.53.2.235
- PubMed https://pubmed.ncbi.nlm.nih.gov/31964470
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Cardiac regeneration with adult stem-cell (ASC) therapy is a promising field to address advanced cardiovascular diseases. In addition, extracellular vesicles (EVs) from ASCs have been implicated in acting as paracrine factors to improve cardiac functions in ASC therapy. In our work, we isolated human cardiac mesenchymal stromal cells (h-CMSCs) by means of three-dimensional organ culture (3D culture) during ex vivo expansion of cardiac tissue, to compare the functional efficacy with human bone-marrow derived mesenchymal stem cells (h-BM-MSCs), one of the actively studied ASCs. We characterized the h-CMSCs as CD90(low), c-kit(negative), CD105(positive) phenotype and these cells express NANOG, SOX2, and GATA4. To identify the more effective type of EVs for angiogenesis among the different sources of ASCs, we isolated EVs which were derived from CMSCs with either nomioxic or hypoxic condition and BM-MSCs. Our in vitro tube-formation results demonstrated that the angiogenic effects of EVs from hypoxia-treated CMSCs (CMSC-Hpx EVs) were greater than the well-known effects of EVs from BM-MSCs (BM-MSC EVs), and these were even comparable to human vascular endothelial growth factor (hVEGF), a potent angiogenic factor. Therefore, we present here that CD90(low)c-kit(negative)CD105(positive) CMSCs under hypoxic conditions secrete functionally superior EVs for in vitro angiogenesis. Our findings will allow more insights understanding myocardial repair.
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