Quantitative and qualitative analysis of autophagy flux using imaging
- 주제(키워드) Autolysosome , Autophagosome , Autophagy , Fluorescence Imaging , Quantitative analysis
- 주제(기타) Biochemistry & Molecular Biology
- 설명문(일반) [Kim, Suree; Choi, Soohee; Kang, Dongmin] Ewha Womans Univ, Fluorescence Core Imaging Ctr, Dept Life Sci, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- OA유형 gold, Green Published
- 발행기관 KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000168773
- 본문언어 영어
- Published As https://dx.doi.org/10.5483/BMBRep.2020.53.5.046
- PubMed https://pubmed.ncbi.nlm.nih.gov/32317089
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
As an intracellular degradation system, autophagy is an essential and defensive cellular program required for cell survival and cellular metabolic homeostasis in response to various stresses, such as nutrient deprivation and the accumulation of damaged organelles. In general, autophagy flux consists of four steps: (1) initiation (formation of phagophore), (2) maturation and completion of autophagosome, (3) fusion of autophagosomes with lysosomes (formation of autolysosome), and (4) degradation of intravesicular components within autolysosomes. The number of genes and reagents that modulate autophagy is increasing. Investigation of their effect on autophagy flux is critical to understanding the roles of autophagy in many physiological and pathological processes. In this review, we summarize and discuss ways to analyze autophagy flux quantitatively and qualitatively with the use of imaging tools. The suggested imaging method can help estimate whether each modulator is an inhibitor or a promoter of autophagy and elucidate the mode of action of specific genes and reagents on autophagy processes.
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