RNA-binding as chaperones of DNA binding proteins from starved cells
- 주제(키워드) E. coli Dps , RNA , Chaperna , Holdase , Anti-aggregation , TEM
- 주제(기타) Biochemistry & Molecular Biology
- 주제(기타) Biophysics
- 설명문(일반) [Park, Chan; Jin, Yoontae; Seong, Baik L.] Yonsei Univ, Dept Biotechnol, 50 Yonsei Ro, Seoul 03722, South Korea; [Park, Chan; Seong, Baik L.] Yonsei Univ, Dept Biomat Sci & Engn, Seoul 03722, South Korea; [Kim, Young Jun] Yonsei Univ, Underwood Int Coll, Life Sci & Biotechnol Dept, Underwood Div, Seoul 03722, South Korea; [Jeong, Hotcherl] Ewha Womans Univ, Dept Pharm, Seoul 03760, South Korea
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 ACADEMIC PRESS INC ELSEVIER SCIENCE
- 발행년도 2020
- URI http://www.dcollection.net/handler/ewha/000000168873
- 본문언어 영어
- Published As https://dx.doi.org/10.1016/j.bbrc.2020.01.121
- PubMed https://pubmed.ncbi.nlm.nih.gov/32007271
초록/요약
a DNA-binding proteins from starved cells (Dps) in Escherichia coli protects DNA from multiple stresses during the stationary phase by forming a stable Dps-DNA complex. In contrast, Dps cannot bind to DNA during the exponential phase and it has not been clear why Dps conditionally binds to DNA depending on the growth phase. In this study, we show that DNA-free Dps in the exponential phase can also bind to RNA and the preemptive binding of RNA precludes DNA from interacting with Dps. The critical role of RNA in modulating the stability and functional competence of Dps and their morphology, leads us to propose a two-state model of Dps in executing stress responses. In the exponential phase, Dps is present predominantly as ribonucleoprotein complex. Under starvation, RNAs are degraded by up-regulated RNases, activating Dps to bind with chromosomal DNAs protecting them from diverse stresses. A dual role of RNA as an inhibitor of DNA binding and chaperone to keep dynamic functional status of Dps would be crucial for operating an immediate protection of chromosomal DNAs on starvation. The holdase-type chaperoning role of RNA in Dps-mediated stress responses would shed light on the role of RNAs as chaperone (Chaperna). (C) 2020 Elsevier Inc. All rights reserved.
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