N-Acetyldopamine derivatives from Periostracum Cicadae and their regulatory activities on Th1 and Th17 cell differentiation
- 주제(키워드) 1H iterative Full Spin Analysis (HiFSA) , CD4+ T cell , Electronic circular dichroism (ECD) calculation , N-acetyldopamine dimers , Periostracum Cicadae
- 등재 SCIE, SCOPUS
- 발행기관 Academic Press Inc.
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000168886
- 본문언어 영어
- Published As https://dx.doi.org/10.1016/j.bioorg.2020.104095
- PubMed https://pubmed.ncbi.nlm.nih.gov/32721777
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Bioassay-guided fractionation of a 90% ethanol extract of Periostracum Cicadae led to the isolation of two new N-acetyldopamine dimers (1a/1b) along with six known dimers (2a/2b, 3a/3b, and 4a/4b) and two monomers (5a/5b); compounds 2a/2b, 4a/4b and 5a/5b were newly isolated from this material. All compounds were isolated as enantiomeric mixtures and each enantiomer was successfully separated by chiral-phase HPLC. The structures including absolute configurations were confirmed by high-resolution electrospray ionization mass spectrometry (HR-ESIMS), 1D/2D nuclear magnetic resonance (NMR) spectroscopy, 1H iterative Full Spin Analysis (HiFSA), and electronic circular dichroism (ECD) spectroscopy. Subsequently, the bioactivities of these isolates were evaluated via CD4+ T cell differentiations, which are critical for immune responses and inflammation. The results revealed that compound 5b was observed to enhance the IFN-γ+ Th1 differentiation, which may have a potential for cancer immunotherapy. © 2020 Elsevier Inc.
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