Simultaneous monitoring of the bioconversion from lysine to glutaric acid by ethyl chloroformate derivatization and gas chromatography-mass spectrometry
- 주제(키워드) Glutaric acid , Metabolites , Gas chromatography-mass spectrometry , Ethyl chloroformate , Mass chromatograms , Analytical method
- 주제(기타) Biochemical Research Methods
- 주제(기타) Biochemistry & Molecular Biology
- 주제(기타) Chemistry, Analytical
- 설명문(일반) [Han, Yeong-Hoon; Choi, Tae-Rim; Park, Ye-Lim; Song, Hun-Suk; Choi, Yong-Keun; Kim, Hyun-Joong; Bhatia, Shashi Kant; Gurav, Ranjit; Yang, Yung-Hun] Konkuk Univ, Coll Engn, Dept Biol Engn, 1 Hwayang Dong, Seoul 05029, South Korea; [Park, Kyungmoon; Park, See-Hyoung] Hongik Univ, Dept Biol & Chem Engn, Sejong Ro 2639, Sejong City 30016, South Korea; [Kim, Wooseong] Ewha Womans Univ Seoul, Coll Pharm, Seoul 03760, South Korea; [Kim, Wooseong] Ewha Womans Univ Seoul, Grad Sch Pharmaceut Sci, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 ACADEMIC PRESS INC ELSEVIER SCIENCE
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000168944
- 본문언어 영어
- Published As https://dx.doi.org/10.1016/j.ab.2020.113688
- PubMed https://pubmed.ncbi.nlm.nih.gov/32194075
초록/요약
Glutaric acid is a precursor of a plasticizer that can be used for the production of polyester amides, ester plasticizer, corrosion inhibitor, and others. Glutaric acid can be produced either via bioconversion or chemical synthesis, and some metabolites and intermediates are produced during the reaction. To ensure reaction efficiency, the substrates, intermediates, and products, especially in the bioconversion system, should be closely monitored. Until now, high performance liquid chromatography (HPLC) has generally been used to analyze the glutaric acid-related metabolites, although it demands separate time-consuming derivatization and non-derivatization analyses. To substitute for this unreasonable analytical method, we applied herein a gas chromatography - mass spectrometry (GC-MS) method with ethyl chloroformate (ECF) derivatization to simultaneously monitor the major metabolites. We determined the suitability of GC-MS analysis using defined concentrations of six metabolites (L-lysine, cadaverine, 5-aminovaleric acid, 2-oxoglutaric acid, glutamate, and glutaric acid) and their mass chromatograms, regression equations, regression coefficient values (R-2), dynamic ranges (mM), and retention times (RT). This method successfully monitored the production process in complex fermentation broth.
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