Optimization of tenocyte lineage-related factors from tonsil-derived mesenchymal stem cells using response surface methodology
- 주제(키워드) Tenocyte , Tonsil-derived mesenchymal stem cells , Bone marrow-derived mesenchymal stem cells , Design of experiments , Response surface methodology
- 주제(기타) Orthopedics
- 설명문(일반) [Kwon, Soon-Sun] Ajou Univ, Coll Nat Sci, Dept Math, Suwon, Gyeonggi, South Korea; [Kim, Hyang; Shin, Sang-Jin] Ewha Womans Univ, Dept Orthopaed Surg, Seoul Hosp, Seoul, South Korea; [Kim, Hyang] Ewha Womans Univ, Sch Med, Ewha Med Res Inst, Seoul, South Korea; [Lee, Seung Yeol] Ewha Womans Univ, Div Mech & Biomed Engn, 52 Ewhayeodae Gil, Seoul 03760, South Korea; [Lee, Seung Yeol] Hanyang Univ, Myongji Hosp, Dept Orthopaed Surg, Coll Med, Seoul, South Korea
- 등재 SCIE, SCOPUS
- OA유형 Green Published, gold, Green Submitted
- 발행기관 BMC
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000169255
- 본문언어 영어
- Published As https://dx.doi.org/10.1186/s13018-020-01623-8
- PubMed https://pubmed.ncbi.nlm.nih.gov/32183870
초록/요약
Background In order to optimize the tenogenic differentiation of mesenchymal stem cells (MSCs), researchers should consider various factors. However, this requires testing numerous experimental settings, which is costly and time-consuming. We aimed to assess the differential effects of transforming growth factor beta-3 (TGF-beta 3) on the tenogenesis of tonsil-derived MSCs (T-MSCs) and bone marrow-derived MSCs (BM-MSCs) using response surface methodology (RSM). Methods Bone marrow and tonsillar tissue were collected from four patients; mononuclear cells were separated and treated with 5 or 10 ng/mL of TGF-beta 3. A full factorial experimental design with a categorical factor of 0 was employed to study the effect of tension based on T-MSCs. Eighty-four trials were fitted with RSM and then used to obtain mathematical prediction models. Results Exposure of T-MSCs and BM-MSCs to TGF-beta 3 increased the expression of scleraxis (SCX), tenomodulin (TNMD), decorin, collagen I, and tenascin C. Expression of most of these factors reached a maximum after 2-3 days of treatment. The model predicted that the values of the tenocyte lineage-related factors assessed would be significantly increased at 2.5 days of culture with 2.7 ng/mL of TGF-beta 3 for T-MSCs and at 2.3 days of culture regardless of TGF-beta 3 concentration for BM-MSCs. Conclusions This study demonstrated that the RSM prediction of the culture time necessary for the tenogenic differentiation of T-MSCs and BM-MSCs under TGF-beta 3 stimulation was similar to the experimentally determined time of peak expression of tenocyte-related mRNAs, suggesting the potential of using the RSM approach for optimization of the culture protocol for tenogenesis of MSCs.
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