Aminopeptidase N Activatable Fluorescent Probe for Tracking Metastatic Cancer and Image-Guided Surgery via in Situ Spraying
- 주제(기타) Chemistry, Multidisciplinary
- 설명문(일반) [Li, Haidong; Chung, Jeewon; Kim, Dayeh; Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nanosci, Seoul 03760, South Korea; [Li, Haidong; Yao, Qichao; Sun, Wen; Shao, Kun; Lu, Yang; Fan, Jiangli; Long, Saran; Du, Jianjun; Peng, Xiaojun] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China; [Sun, Wen; Shao, Kun; Fan, Jiangli; Long, Saran; Du, Jianjun; Peng, Xiaojun] Dalian Univ Technol, Shenzhen Res Inst, Dalian 116024, Peoples R China; [Li, Yueqing] Dalian Univ Technol, Sch Pharmaceut Sci & Technol, Dalian 116024, Peoples R China; [Wang, Jingyun] Dalian Univ Technol, Sch Lifc Sci & Biotechnol, Dalian 116024, Peoples R China
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000169500
- 본문언어 영어
- Published As https://dx.doi.org/10.1021/jacs.0c01365
- PubMed https://pubmed.ncbi.nlm.nih.gov/32167306
초록/요약
The recurrence of malignant tumors is mostly caused by incompleted surgical resection. Especially, it is difficult for surgeons to detect and accurately remove metastatic tumors by predominantly using visual examination and palpation owing to the lack of effective means to specifically distinguish the boundary range between normal and tumor tissues. Thus, the development of activated fluorescent probe with superior tumor-to-normal (T/N) tissue ratios is particularly urgent in clinics. In view of CD13/aminopeptidase N (APN) regarded as a cancer-specific biomarker, mediating with progression, invasion, and migration of malignant tumor, herein, we reported an APN-responsive fluorescent probe YH-APN and demonstrated its application to distinguish cancer cells. Through in situ spraying manner, fluorescent superior tumor-to-normal (T/N) tissue ratios (subcutaneous transplantation tumor, 13.86; hepatic metastasis, 4.42 and 6.25; splenic metastasis, 4.99) were achieved. More importantly, we have demonstrated the ability to image metastasis tumor tissue less than 1 mm in diameter, highlighting the potential for this probe to be used as a tool in surgical resection. This research may spur the use of enzyme-activatable fluorescent probes for the progress of tumor diagnosis and image-guided surgery (IGS).
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