Two-photon imaging of hydrogen polysulfides in living cells and hippocampal tissues
- 주제(키워드) Cell imaging , Fluorescent probe , Hydrogen polysulfides , Two-photon
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier B.V.
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000169507
- 본문언어 영어
- Published As https://dx.doi.org/10.1016/j.snb.2020.128564
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Hydrogen polysulfide (H2Sn) is an emerging topic of research because it plays a critical role in the pathological and physiological activities of an organism. Therefore, visual monitoring of H2Sn activity in living cells is critical. In our work, a two-photon fluorescent probe NI-H2S2 based on a naphthalimide skeleton was developed as a molecular tool for detecting hydrogen polysulfides in solution and live HeLa cells. NI-H2S2 showed rapid response and high sensitivity. Notably, hydrogen polysulfides in hippocampal tissue of mice could also be tracked using the probe under a two-photon fluorescence microscope. Therefore, this probe NI-H2S2 is expected to serve as a pragmatic tool for studying the physiological roles of hydrogen polysulfides in living cells and in vivo. © 2020 Elsevier B.V.
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