Whole-Cell Photoenzymatic Cascades to Synthesize Long-Chain Aliphatic Amines and Esters from Renewable Fatty Acids
- 주제(키워드) Biocatalysis , fatty acids , long-chain aliphatic amines , photodecarboxylase , whole-cell biocatalysis
- 등재 SCIE, SCOPUS
- OA유형 Green Published
- 발행기관 Wiley-VCH Verlag
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000169525
- 본문언어 영어
- Published As https://dx.doi.org/10.1002/anie.201915108
- PubMed https://pubmed.ncbi.nlm.nih.gov/31957098
초록/요약
Long-chain aliphatic amines such as (S,Z)-heptadec-9-en-7-amine and 9-aminoheptadecane were synthesized from ricinoleic acid and oleic acid, respectively, by whole-cell cascade reactions using the combination of an alcohol dehydrogenase (ADH) from Micrococcus luteus, an engineered amine transaminase from Vibrio fluvialis (Vf-ATA), and a photoactivated decarboxylase from Chlorella variabilis NC64A (Cv-FAP) in a one-pot process. In addition, long chain aliphatic esters such as 10-(heptanoyloxy)dec-8-ene and octylnonanoate were prepared from ricinoleic acid and oleic acid, respectively, by using the combination of the ADH, a Baeyer–Villiger monooxygenase variant from Pseudomonas putida KT2440, and the Cv-FAP. The target compounds were produced at rates of up to 37 U g−1 dry cells with conversions up to 90 %. Therefore, this study contributes to the preparation of industrially relevant long-chain aliphatic chiral amines and esters from renewable fatty acid resources. © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
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