An Activatable AIEgen Probe for High-Fidelity Monitoring of Overexpressed Tumor Enzyme Activity and Its Application to Surgical Tumor Excision
- 주제(키워드) aggregation-induced emission , AIEgens , enzyme activity , nanoprobes , tumor detection
- 주제(기타) Chemistry, Multidisciplinary
- 설명문(일반) [Li, Haidong; Kim, Dayeh; Chung, Jeewon; Baek, Gain; Wu, Xiaofeng; Hillman, Prima Fitria; Lee, Eun Young; Nam, Sang-Jip; Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nanosci, Seoul 03760, South Korea; [Yao, Qichao; Xu, Feng; Ge, Haoying; Fan, Jiangli; Peng, Xiaojun] Dalian Univ Technol, State Key Lab Fine Chem, 2 Linggong Rd, Dalian 116024, Peoples R China; [Li, Yueqing] Dalian Univ Technol, Sch Pharmaceut Sci & Technol, 2 Linggong Rd, Dalian 116024, Peoples R China; [Wang, Jingyun] Dalian Univ Technol, Sch Life Sci & Biotechnol, 2 Linggong Rd, Dalian 116024, Peoples R China; [Fan, Jiangli; Peng, Xiaojun] Dalian Univ Technol Shenzhen, Res Inst, Gaoxin South Fourth Rd, Shenzhen 518057, Peoples R China
- 등재 SCIE, SCOPUS
- 발행기관 WILEY-V C H VERLAG GMBH
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000169549
- 본문언어 영어
- Published As https://dx.doi.org/10.1002/anie.202001675
- PubMed https://pubmed.ncbi.nlm.nih.gov/32155310
초록/요약
Monitoring fluctuations in enzyme overexpression facilitates early tumor detection and excision. An AIEgen probe (DQM-ALP) for the imaging of alkaline phosphatase (ALP) activity was synthesized. The probe consists of a quinoline-malononitrile (QM) core decorated with hydrophilic phosphate groups as ALP-recognition units. The rapid liberation of DQM-OH aggregates in the presence of ALP resulted in aggregation-induced fluorescence. The up-regulation of ALP expression in tumor cells was imaged using DQM-ALP. The probe permeated into 3D cervical and liver tumor spheroids for imaging spatially heterogeneous ALP activity with high spatial resolution on a two-photon microscopy platform, providing the fluorescence-guided recognition of sub-millimeter tumorigenesis. DQM-ALP enabled differentiation between tumor and normal tissue ex vivo and in vivo, suggesting that the probe may serve as a powerful tool to assist surgeons during tumor resection.
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