Biosynthesis of polyhydroxyalkanoates from sucrose by metabolically engineered Escherichia coli strains
- 주제(키워드) Escherichia coli , Sucrose , Polyhydroxyalkanoates
- 주제(기타) Biochemistry & Molecular Biology
- 주제(기타) Chemistry, Applied
- 주제(기타) Polymer Science
- 설명문(일반) [Sohn, Yu Jung; Baritugo, Kei-Anne; Song, Hye Min; Jo, Seo Young; Park, Su Kyeong; Park, Si Jae] Ewha Womans Univ, Div Chem Engn & Mat Sci, 52 Ewhayeodae Gil, Seoul 03760, South Korea; [Kim, Hee Taek; Ryu, Mi Hee; Kang, Kyoung Hee; Joo, Jeong Chan] Korea Res Inst Chem Technol, Adv Convergent Chem Div, Biobased Chem Res Ctr, POB 107,141 Gajeong Ro, Daejeon 34602, South Korea; [Kim, Hoyong] Korea Res Inst Chem Technol, Adv Convergent Chem Div, Biobased Chem Res Ctr, Jongga Ro, Ulsan 44429, South Korea; [Kim, You Jin] Myongji Univ, Dept Environm Engn & Energy, Yongin 17058, Gyeonggi Do, South Korea; [Choi, Jong-il] Chonnam Natl Univ, Dept Biotechnol & Engn, Interdisciplinary Program Bioenergy & Biomat, 77 Yongbong Ro, Gwangju 61186, South Korea
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER
- 발행년도 2020
- URI http://www.dcollection.net/handler/ewha/000000169569
- 본문언어 영어
- Published As https://dx.doi.org/10.1016/j.ijbiomac.2020.01.254
- PubMed https://pubmed.ncbi.nlm.nih.gov/32001289
초록/요약
Sucrose utilization has been established in Escherichia coil strains by expression of Mannheimia succiniciproducens beta-fructofuranosidase (SacC), which hydrolyzes sucrose into glucose and fructose. Recombinant E. coil strains that can utilize sucrose were examined for their abilities to produce poly(3-hydroxybutyrate) IP( 3HB)1 and poly(3-hydroxybutyrate-co-lactate) (P(3HB-co-LA)] from sucrose. When recombinant E. coil strains expressing Ralstonia eutropha PhaCAB and SacC were cultured in MR medium containing 20 g/L of sucrose, all recombinant E. coil strains could produce P(3HB) from sucrose. Also, recombinant E. coli strains expressing Pseudomonas sp. MBEL 6-19 PhaC1437, Clostridium propionicum Pct540, R. eutropha PhaAB enzymes along with SacC could produce P (3H13-co-1A) from sucrose. Among the examined E. coli strains, recombinant E. coil XL1-Blue produced the highest contents of P(3HB) (53.60 +/- 255 wt%) and P(3HB-co-LA) (29.44 +/- 039 wt%). In the batch fermentations, recombinant E. coil XL1-Blue strains completely consumed 20 g/L of sucrose as the sole carbon source and supported the production of 3.76 g/L of P(3HB) and 1.82 g/L of P(3HB-co-LA) with 38.21 wt%P(3HB) and 20.88 wt% P(3HB-co-LA) contents, respectively. Recombinant E. coli strains developed in this study can be used to establish a costefficient biorefinery for the production of polyhydroxyalkanoates (PHAs) from sucrose, which is an abundant and inexpensive carbon source. (C) 2020 Elsevier B.V. All rights reserved.
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