Oxygenated polycyclic aromatic hydrocarbons from ambient particulate matter induce electrophysiological instability in cardiomyocytes
- 주제(키워드) Ambient particulate matter , Oxygenated polycyclic aromatic hydrocarbons , Electrophysiological instability , Cardiomyocytes , Reactive oxygen species
- 주제(기타) Toxicology
- 설명문(일반) [Ju, Sujin; Lim, Leejin; Song, Heesang] Chosun Univ, Dept Biomat, Grad Sch, Gwangju 61452, South Korea; [Lim, Leejin] Chosun Univ, Canc Mutat Res Ctr, Gwangju 61452, South Korea; [Jiao, Han-Yi; Choi, Seok; Jun, Jae Yeoul] Chosun Univ, Dept Physiol, Sch Med, Gwangju 61452, South Korea; [Ki, Young-Jae; Choi, Dong-Hyun] Chosun Univ, Dept Internal Med, Sch Med, Gwangju 61452, South Korea; [Lee, Ji Yi] Ewha Womans Univ, Dept Environm Sci & Engn, Seoul 03760, South Korea; [Song, Heesang] Chosun Univ, Sch Med, Dept Biochem & Mol Biol, Gwangju 61452, South Korea
- 등재 SCIE, SCOPUS
- OA유형 gold, Green Published
- 발행기관 BMC
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000169586
- 본문언어 영어
- Published As https://dx.doi.org/10.1186/s12989-020-00351-5
- PubMed https://pubmed.ncbi.nlm.nih.gov/32527278
초록/요약
Background Epidemiologic studies have suggested that elevated concentrations of particulate matter (PM) are strongly associated with an increased risk of developing cardiovascular diseases, including arrhythmia. However, the cellular and molecular mechanisms by which PM exposure causes arrhythmia and the component that is mainly responsible for this adverse effect remains to be established. In this study, the arrhythmogenicity of mobilized organic matter from two different types of PM collected during summer (SPM) and winter (WPM) seasons in the Seoul metropolitan area was evaluated. In addition, differential effects between polycyclic aromatic hydrocarbons (PAHs) and oxygenated PAHs (oxy-PAHs) on the induction of electrophysiological instability were examined. Results We extracted the bioavailable organic contents of ambient PM, measuring 10 mu m or less in diameter, collected from the Seoul metropolitan area using a high-volume air sampler. Significant alterations in all factors tested for association with electrophysiological instability, such as intracellular Ca(2+)levels, reactive oxygen species (ROS) generation, and mRNA levels of the Ca2+-regulating proteins, sarcoplasmic reticulum Ca(2+)ATPase (SERCA2a), Ca2+/calmodulin-dependent protein kinase II (CaMK II), and ryanodine receptor 2 (RyR2) were observed in cardiomyocytes treated with PM. Moreover, the alterations were higher in WPM-treated cardiomyocytes than in SPM-treated cardiomyocytes. Three-fold more oxy-PAH concentrations were observed in WPM than SPM. As expected, electrophysiological instability was induced higher in oxy-PAHs (9,10-anthraquinone, AQ or 7,12-benz(a) anthraquinone, BAQ)-treated cardiomyocytes than in PAHs (anthracene, ANT or benz(a) anthracene, BaA)-treated cardiomyocytes; oxy-PAHs infusion of cells mediated by aryl hydrocarbon receptor (AhR) was faster than PAHs infusion. In addition, ROS formation and expression of calcium-related genes were markedly more altered in cells treated with oxy-PAHs compared to those treated with PAHs. Conclusions The concentrations of oxy-PAHs in PM were found to be higher in winter than in summer, which might lead to greater electrophysiological instability through the ROS generation and disruption of calcium regulation.
more