Flow Homogenization Enables a Massively Parallel Fluidic Design for High-Throughput and Multiplexed Cell Isolation
- 주제(키워드) flow homogenization , magnetic separation , microfluidics , multiplexed cell separation , rare cell isolation
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- OA유형 Green Accepted
- 발행기관 Wiley-Blackwell
- 발행년도 2020
- URI http://www.dcollection.net/handler/ewha/000000169601
- 본문언어 영어
- Published As https://dx.doi.org/10.1002/admt.201900960
- PubMed https://pubmed.ncbi.nlm.nih.gov/33072854
초록/요약
Microfluidic devices are widely used for applications such as cell isolation. Currently, the most common method to improve throughput for microfluidic devices involves fabrication of multiple, identical channels in parallel. However, this “numbering up” only occurs in one dimension, thereby limiting gains in volumetric throughput. In contrast, macrofluidic devices permit high volumetric flow rates but lack the finer control of microfluidics. Here, it is demonstrated how a micropore array design enables flow homogenization across a magnetic cell capture device, thus creating a massively parallel series of microscale flow channels with consistent fluidic and magnetic properties, regardless of spatial location. This design enables scaling in two dimensions, allowing flow rates exceeding 100 mL h−1 while maintaining >90% capture efficiencies of spiked lung cancer cells from blood in a simulated circulating tumor cell system. Additionally, this design facilitates modularity in operation, which is demonstrated by combining two different devices in tandem for multiplexed cell separation in a single pass with no additional cell losses from processing. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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