Phenol-Soluble Modulin-Mediated Aggregation of Community-Associated Methicillin-Resistant Staphylococcus Aureus in Human Cerebrospinal Fluid
- 주제(키워드) community-associated methicillin-resistant staphylococcus , aureus , phenol-soluble modulins , cerebrospinal fluid , bacterial aggregation
- 주제(기타) Cell Biology
- 설명문(일반) [Kim, Deok-ryeong] Eulji Univ, Nowon Eulji Med Ctr, Dept Neurosurg, Seoul 01830, South Korea; [Lee, Yeonhee; Kim, Hyeon-kyeong; Joo, Hwang-Soo] Duksung Womens Univ, Coll Sci & Technol, Grad Sch Interdisciplinary Convergence Funct Mat, Seoul 01369, South Korea; [Kim, Wooseong] Ewha Womans Univ, Coll Pharm, Grad Sch Pharmaceut Sci, Seoul 03760, South Korea; [Kim, Yun-Gon] Soongsil Univ, Dept Chem Engn, Seoul 06978, South Korea; [Yang, Yung-Hun] Konkuk Univ, Coll Engn, Dept Biol Engn, Seoul 05029, South Korea; [Kim, Jae-Seok] Hallym Univ, Kangdong Sacred Heart Hosp, Dept Lab Med, Coll Med, Seoul 05355, South Korea; [Joo, Hwang-Soo] Duksung Womens Univ, Coll Sci & Technol, Dept Biotechnol, Seoul 01369, South Korea
- 등재 SCIE, SCOPUS
- OA유형 Green Published, gold
- 발행기관 MDPI
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000172083
- 본문언어 영어
- Published As https://dx.doi.org/10.3390/cells9030788
- PubMed https://pubmed.ncbi.nlm.nih.gov/32214032
초록/요약
Phenol-soluble modulins (PSMs) are major determinants of Staphylococcus aureus virulence and their increased production in community-associated methicillin-resistant S. aureus (CA-MRSA) likely contributes to the enhanced virulence of MRSA strains. Here, we analyzed the differences in bacterial cell aggregation according to PSM presence in the specific human cerebrospinal fluid (CSF) environment. CSF samples from the intraventricular or lumbar intrathecal area of each patient and tryptic soy broth media were mixed at a 1:1 ratio, inoculated with WT and PSM-deleted mutants (Delta psm) of the CA-MRSA strain, USA300 LAC, and incubated overnight. Cell aggregation images were acquired after culture and image analysis was performed. The cell aggregation ratio in WT samples differed significantly between the two sampling sites (intraventricular: 0.2% vs. lumbar intrathecal: 6.7%, p < 0.001). The cell aggregation ratio in Delta psm samples also differed significantly between the two sampling sites (intraventricular: 0.0% vs. lumbar intrathecal: 1.2%, p < 0.001). Division of the study cases into two groups according to the aggregated area ratio (WT/Delta psm; group A: ratio of >= 2, group B: ratio of < 2) showed that the median aggregation ratio value differed significantly between groups A and B (5.5 and 0, respectively, p < 0.001). The differences in CSF distribution and PSM presence within the specific CSF environment are significant factors affecting bacterial cell aggregation.
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