Assessment of the purity of emodin by quantitative nuclear magnetic resonance spectroscopy and mass balance
- 주제(키워드) Emodin , HPLC-UV , Mass balance , QNMR
- 등재 SCOPUS, KCI등재
- 발행기관 Korean Society of Pharmacognosy
- 발행년도 2019
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000172159
- 본문언어 영어
- Published As https://dx.doi.org/10.20307/nps.2019.25.3.222
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Quantitative nuclear magnetic resonance (qNMR) is a well-established method adopted by international pharmacopoeia for quantitative and purity analyses. Emodin is a type of anthraquinone, well known as the main active component of Fabaceae, Polygonaceae and Rhamnaceae. Purity analysis of emodin is usually performed by using the high-performance liquid chromatography (HPLC)-UV method. However, it cannot detect impurities such as salts, volatile matter, and trace elements. Using the qNMR method, it is possible to determine the compound content as well as the nature of the impurities. Several experimental parameters were optimized for the quantification, such as relaxation delay, spectral width, number of scans, temperature, pulse width, and acquisition time. The method was validated, and the results of the qNMR method were compared with those obtained by the HPLC and mass balance analysis methods. The qNMR method is specific, rapid, simple, and therefore, a valuable and reliable method for the purity analysis of emodin. © 2019, Korean Society of Pharmacognosy. All rights reserved.
more