Assay Systems for Profiling Deubiquitinating Activity
- 주제(키워드) activity-based probes , cell permeability , deubiquitination assay , Ub-probes
- 등재 SCIE, SCOPUS
- OA유형 gold, Green Published
- 발행기관 NLM (Medline)
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000172285
- 본문언어 영어
- Published As https://dx.doi.org/10.3390/ijms21165638
- PubMed https://pubmed.ncbi.nlm.nih.gov/32781716
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Deubiquitinating enzymes regulate various cellular processes, particularly protein degradation, localization, and protein-protein interactions. The dysregulation of deubiquitinating enzyme (DUB) activity has been linked to several diseases; however, the function of many DUBs has not been identified. Therefore, the development of methods to assess DUB activity is important to identify novel DUBs, characterize DUB selectivity, and profile dynamic DUB substrates. Here, we review various methods of evaluating DUB activity using cell lysates or purified DUBs, as well as the types of probes used in these methods. In addition, we introduce some techniques that can deliver DUB probes into the cells and cell-permeable activity-based probes to directly visualize and quantify DUB activity in live cells. This review could contribute to the development of DUB inhibitors by providing important information on the characteristics and applications of various probes used to evaluate and detect DUB activity in vitro and in vivo.
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