A fluorescent ESIPT-based benzimidazole platform for the ratiometric two-photon imaging of ONOO(-)in vitroandex vivo
- 주제(기타) Chemistry, Multidisciplinary
- 설명문(일반) [Odyniec, Maria L.; Gardiner, Jordan E.; Webb, Emily C.; Bull, Steven D.; James, Tony D.] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England; [Park, Sang-Jun; Kim, Hwan Myung] Ajou Univ, Dept Chem, Suwon 16499, South Korea; [Sedgwick, Adam C.] Univ Texas Austin, Dept Chem, 105 E,24th St,A5300, Austin, TX 78712 USA; [Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea
- 등재 SCIE, SCOPUS
- OA유형 Green Published, gold
- 발행기관 ROYAL SOC CHEMISTRY
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000172347
- 본문언어 영어
- Published As https://dx.doi.org/10.1039/d0sc02347g
- PubMed https://pubmed.ncbi.nlm.nih.gov/33033609
초록/요약
In this work, we have developed an ESIPT-based benzimidazole platform (MO-E1andMO-E2) for the two-photon cell imaging of ONOO(-)and a potential ONOO--activated theranostic scaffold (MO-E3). Each benzimidazole platform,MO-E1-3, were shown to rapidly detect ONOO(-)at micromolar concentrations (LoD = 0.28 mu M, 6.53 mu M and 0.81 mu M respectively). The potential theranosticMO-E3was shown to release the parent fluorophore and drug indomethacin in the presence of ONOO(-)but unfortunately did not perform wellin vitrodue to low solubility. Despite this, the parent scaffoldMO-E2demonstrated its effectiveness as a two-photon imaging tool for the ratiometric detection of endogenous ONOO(-)in RAW264.7 macrophages and rat hippocampus tissue. These results demonstrate the utility of this ESIPT benzimidazole-based platform for theranostic development and bioimaging applications.
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