Haloplanus rubicundus sp. nov., an extremely halophilic archaeon isolated from solar salt
- 주제(키워드) Haloarchaea , Haloplanus rubicundus , Solar salt , Polyphasic taxonomy
- 주제(기타) Biotechnology & Applied Microbiology
- 주제(기타) Microbiology
- 설명문(일반) [Kim, Yeon Bee; Song, Hye Seon; Lee, Se Hee; Roh, Seong Woon] World Inst Kimchi, Microbiol & Funct Res Grp, Gwangju 61755, South Korea; [Kim, Yeon Bee; Song, Hye Seon; Rhee, Jin-Kyu] Ewha Womans Univ, Dept Food Sci & Engn, Seoul 03760, South Korea; [Kim, Joon Yong; Bae, Jin-Woo] Kyung Hee Univ, Dept Biol, Seoul 02447, South Korea; [Shin, Na-Ri] Korea Res Inst Biosci & Biotechnol, Biol Resource Ctr, Jeollabuk Do 56212, South Korea; [Myoung, Jinjong] Chonbuk Natl Univ, Korea Zoonosis Res Inst, Jeollabuk Do 54531, South Korea; [Lee, Ki-Eun; Cha, In-Tae] Natl Inst Biol Resources, Microorganism Resources Div, Incheon 22689, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER GMBH
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000172379
- 본문언어 영어
- Published As https://dx.doi.org/10.1016/j.syapm.2020.126085
- PubMed https://pubmed.ncbi.nlm.nih.gov/32381322
초록/요약
Two extremely halophilic archaea strains, CBA1112(T) and CBA1113, were isolated from solar salt in Korea. The genome sizes and G + C content of CBA1112(T) and CBA1113 were 3.77 and 3.53 Mb, and 66.0 and 66.5 mol%, respectively. Phylogenetic analysis based on closely related taxa and environmental Haloplanus sequences indicated that both CBA1112(T) and CBA1113 strains are grouped within the genus Haloplanus. OrthoANI and in silico DNA-DNA hybridization values were below the species delineation threshold. Pan-genomic analysis showed that the two novel strains and four reference strains had 6203 pan-orthologous groups in total. Six Halop lanus strains shared 1728 core pan-genome orthologous groups, which were mainly associated with amino acid transport and metabolism and translation, ribosomal structure and biogenesis categories, and amino acid metabolism and carbohydrate metabolism related categories. The novel strain-specific pan-genome orthologous groups were mainly involved with replication, recombination and repair category and replication and repair pathway or amino acid metabolism pathway. Cells of both strains were Gram-negative and pleomorphic, and colonies were red-pigmented. The major polar lipids of both strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, and one glycolipid, sulfated mannosyl glucosyl diether. Based on genomic, phylogenetic, phenotypic, and chemotaxonomic features, strains CBA1112(T) and CBA1113 are described as novel species of the genus Haloplanus. Thus, we propose the name Haloplanus rubicundus sp. nov. The type strain is CBA1112(T) (=KCCM 43224(T) = JCM 30475(T)). (C) 2020 Elsevier GmbH. All rights reserved.
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