Increased Production of omega-Hydroxynonanoic Acid and alpha,omega-Nonanedioic Acid from Olive Oil by a Constructed Biocatalytic System
- 주제(키워드) omega-hydroxynonanoic acid , alpha , omega-nonanedioic acid , olive oil , biotransformation , resin binding , solubility decrease , substrate feeding
- 주제(기타) Agriculture, Multidisciplinary
- 주제(기타) Chemistry, Applied
- 주제(기타) Food Science & Technology
- 설명문(일반) [Kang, Su-Hwan; Kim, Tae-Hun; Oh, Deok-Kun] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 05029, South Korea; [Park, Jin-Byung] Ewha Womans Univ, Dept Food Sci & Engn, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000174522
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acs.jafc.0c03255
- PubMed https://pubmed.ncbi.nlm.nih.gov/32786834
초록/요약
omega-Hydroxynonanoic acid and alpha,omega-nonanedioic acid are used for synthesizing diverse chemicals. Although biological methods are developed, their concentrations are low due to the toxicity of high concentrations of the hydrophobic chemicals toward biocatalysts. Here, we constructed a biocatalytic system with high productivity by adding an adsorbent resin and a strong base anion-exchange resin, reducing the solubility of omega-hydroxynonanoic acid and alpha,omega-nonanedioic acid, feeding omega-hydroxynonanoic acid, and introducing a cofactor regeneration system. The constructed biocatalytic system converted 300 mM (83.9 g L-1) and 154 mM (43.5 g L-1) oleic acid in the olive oil hydrolysate obtained after resin extraction, which were derived from 110 and 54 g L-1 olive oil, respectively, into 202 mM (35.2 g L-1) omega-hydroxynonanoic acid and 103 mM (19.4 g L-1) alpha,omega-nonanedioic acid, which are 21- and 24-fold higher values than the previously reported results, respectively. This study may contribute to the industrial biosynthesis of omega-hydroxynonanoic acid and alpha,omega-nonanedioic acid from olive oil.
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