Utilization of Noxious Weed Water Hyacinth Biomass as a Potential Feedstock for Biopolymers Production: A Novel Approach
- 주제(키워드) water hyacinth biomass , peracetic acid pretreatment , alkaline pretreatment , poly(3-hydroxybutyrate) , Ralstonia eutropha , enzymatic hydrolysis
- 주제(기타) Polymer Science
- 설명문(일반) [Saratale, Rijuta Ganesh] Dongguk Univ Seoul, Res Inst Biotechnol & Med Converged Sci, Goyang Si 10326, Gyeonggido, South Korea; [Cho, Si-Kyung; Ghodake, Gajanan S.] Dongguk Univ, Dept Biol & Environm Sci, Goyang Si 10326, Gyeonggido, South Korea; [Shin, Han-Seung; Saratale, Ganesh Dattatraya; Park, Yooheon] Dongguk Univ Seoul, Dept Food Sci & Biotechnol, Goyang Si 10326, Gyeonggido, South Korea; [Lee, Hee-Seok] Chung Ang Univ, Dept Food Sci & Technol, Anseong 17546, South Korea; [Bharagava, Ram Naresh] Babasaheb Bhimrao Ambedkar Univ, Dept Microbiol, Sch Environm Sci, Vidya Vihar 226025, Uttar Pradesh, India; [Kim, Dong-Su] Ewha Womans Univ, Dept Environm Sci & Engn, Seoul 120750, South Korea
- 등재 SCIE, SCOPUS
- OA유형 Green Published, gold
- 발행기관 MDPI
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000174595
- 본문언어 영어
- Published As http://dx.doi.org/10.3390/polym12081704
- PubMed https://pubmed.ncbi.nlm.nih.gov/32751380
초록/요약
This study aims to utilize a noxious weed water hyacinth biomass (WH) for polyhydroxybutyrate (PHB) production. Alkaline and peracetic acid pretreatment was employed for the hydrolysis of WH and consequently enzymatic saccharification to produce fermentable sugars for PHB production. The pretreatment competence was determined using various operational parameters. By applying ambient conditions, alkaline pretreatment gave higher lignin removal of 65.0%, with 80.8% hydrolysis yield, and on enzyme hydrolysis (40 FPU/g of dry WH), produced total reducing sugar of about 523 mg/g of WH. The resulted WH enzymatic hydolysates were evaluated for the production of PHB byRalstonia eutropha(ATCC 17699). The WH hydrolysates cultivation was compared to synthetic hydrolysates that contain a similar carbon composition in terms of bacterial growth and PHB synthesis. The effects of various supplements to enhance PHB production were estimated. Supplementation of corn steep liquor (CSL) as a cheap nitrogen source with WH hydrolysates favored a higher amount of PHB synthesis (73%), PHB titer of 7.30 g/L and PHB yield of 0.429 g/g of reducing sugar. Finally, using standard analytical tools, the physical and thermal characteristics of the extracted PHB were evaluated. The findings revealed WH was a promising and technically feasible option for transforming biomass into sustainable biopolymer conversion on a large scale.
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