Exploiting Fruit Waste Grape Pomace for Silver Nanoparticles Synthesis, Assessing Their Antioxidant, Antidiabetic Potential and Antibacterial Activity Against Human Pathogens: A Novel Approach
- 주제(키워드) grape pomace , silver nanoparticles , cytoplasmic leakages , antidiabetic activity , antioxidant potential
- 주제(기타) Nanoscience & Nanotechnology
- 주제(기타) Materials Science, Multidisciplinary
- 설명문(일반) [Saratale, Ganesh Dattatraya; Shin, Han-Seung] Dongguk Univ, Dept Food Sci & Biotechnol, Seoul 10326, South Korea; [Saratale, Rijuta Ganesh; Kim, Do-Yeong] Dongguk Univ, Res Inst Biotechnol & Med Converged Sci, Seoul 10326, South Korea; [Kim, Dong-Su] Ewha Womans Univ, Dept Environm Sci & Engn, Seoul 120750, South Korea
- 등재 SCIE, SCOPUS
- OA유형 gold, Green Published
- 발행기관 MDPI
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000174602
- 본문언어 영어
- Published As http://dx.doi.org/10.3390/nano10081457
- PubMed https://pubmed.ncbi.nlm.nih.gov/32722404
초록/요약
Grape pomace, a most abundant and renewable wine industry waste product was utilized as a suitable reducing, capping, and stabilizing biomolecules for green synthesis of silver nanoparticles (AgNPs). The physicochemical properties of biosynthesized grape pomace extract (GPE)-AgNPs were duly appraised via UV-Visible spectroscopy, X-ray diffractometer (XRD), Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy. The analytical studies revealed that the GPE-AgNPs were well formed and stable in nature. The functional groups of organic molecules of GPE are present on the surface of AgNPs with average NPs diameter in the range of 20-35 nm. GPE-AgNPs exhibited significant free radical scavenging activity mainly DPPH radical (IC50, 50.0 +/- 2.25 mu g/mL) and ABTS radical (IC50, 38.46 +/- 1.14 mu g/mL). Additionally, the synthesized AgNPs showed noticeable inhibition of carbohydrate hydrolyzing enzymes mainly, alpha-amylase (IC50, 60.2 +/- 2.15 mu g/mL) and alpha-glucosidase (IC50, 62.5 +/- 2.75 mu g/mL). The GPE fabricated AgNPs showed noteworthy antibacterial potential against infectious bacteria viz.,Escherichia coliandStaphylococcus aureus. The reaction mechanism of antibacterial activity was studied by measuring the bacterial cell membrane breakage and cytoplasmic contents, mainly, nucleic acid, proteins, and reducing sugar. Therefore, this research attempt illustrated the potential of GPE as a novel source intended for the biosynthesis of AgNPs that may open up new horizons in the field of nanomedicine.
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