초록/요약

Challenges and opportunities: • In situ real-time fluorescence imaging is an extremely useful method for researchers to observe the dynamics of enzymes and their interactions with other species in the biosystem • Developing new methods with high binding efficiency toward enzyme for in situ real-time imaging enzyme remains a great challenge • Enzyme- and photoactivatable fluorescent sensors bearing a diazoindanone moiety may prove to be a promising tool for in situ real-time imaging with high resolution of enzymes © 2020 Elsevier Inc.Enzymes play a key role in many bioprocesses associated with diseases, and observation of enzyme dynamics in living bodies has become meaningful for understanding enzyme function. Thus, in situ real-time fluorescence imaging can prove to be a powerful tool for monitoring enzyme dynamics, enabling detailed understanding of changes in the activity and function of enzymes. This perspective summarizes the existing methods for in situ real-time fluorescence imaging of enzymes in vivo and discusses remaining challenges. This perspective is intended to encourage chemists and chemical engineers to develop additional methods for the in situ real-time monitoring of enzymes in complex biosystems. © 2020 Elsevier Inc.Enzymes, many of which have been identified as vital biomarkers of cancers, play critical roles in many bioprocesses. In situ real-time fluorescence imaging of enzymes has, thus, become an important method for researchers to observe the dynamics of enzymes and their interactions with other species in biosystems. Although several excellent methods have been developed, many of their performance aspects, including their signal-to-noise ratio, sensitivity, and selectivity, require further improvement. This perspective mainly summarizes developed methods to realize the in situ real-time fluorescence imaging of enzymes to better elucidate the dynamics and biofunctions of enzymes. © 2020 Elsevier Inc.