Physiological involvement of presynaptic L-type voltage-dependent calcium channels in GABA release of cerebellar molecular layer interneurons
- 주제(키워드) calcium channels , calcium imaging , cerebellum , synapse
- 주제(기타) Biochemistry & Molecular Biology
- 주제(기타) Neurosciences
- 설명문(일반) [Rey, Stephanie; Llano, Isabel; Collin, Thibault] Univ Paris, St Peres Paris Inst Neurociences CNRS UMR 8003, 45 Rue St Peres, F-75006 Paris, France; [Maton, Gilliane] Univ Paris, CNRS, Inst Jacques Monod, Batiment Buffon, Paris, France; [Satake, Shin'Ichiro] Natl Inst Physiol Sci NIPS, Dept Infornat Physiol, Okazaki, Aichi, Japan; [Kang, Soosung; Silverman, Richard B.] Northwestern Univ, Dept Chem, Chem Life Proc Inst, Evanston, IL USA; [Surmeier, Dalton James] Northwestern Univ, Dept Physiol, Feinberg Sch Med, Chicago, IL 60611 USA; [Kang, Soosung] Ewha Womans Univ, Coll Pharm, Seoul, South Korea
- 등재 SCIE, SCOPUS
- OA유형 Green Submitted
- 발행기관 WILEY
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000174949
- 본문언어 영어
- Published As http://dx.doi.org/10.1111/jnc.15100
- PubMed https://pubmed.ncbi.nlm.nih.gov/32491217
초록/요약
While high threshold voltage-dependent Ca2+ channels (VDCCs) of the N and P/Q families are crucial for evoked neurotransmitter release in the mammalian CNS, it remains unclear to what extent L-type Ca2+ channels (LTCCs), which have been mainly considered as acting at postsynaptic sites, participate in the control of transmitter release. Here, we investigate the possible role of LTCCs in regulating GABA release by cerebellar molecular layer interneurons (MLIs) from rats. We found that BayK8644 (BayK) markedly increases mIPSC frequency in MLIs and Purkinje cells (PCs), suggesting that LTCCs are expressed presynaptically. Furthermore, we observed (1) a potentiation of evoked IPSCs in the presence of BayK, (2) an inhibition of evoked IPSCs in the presence of the LTCC-specific inhibitor Compound 8 (Cp8), and (3) a strong reduction of mIPSC frequency by Cp8. BayK effects are reduced by dantrolene, suggesting that ryanodine receptors act in synergy with LTCCs. Finally, BayK enhances presynaptic AP-evoked Ca2+ transients and increases the frequency of spontaneous axonal Ca2+ transients observed in TTX. Taken together, our data demonstrate that LTCCs are of primary importance in regulating GABA release by MLIs.
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