Camphene Attenuates Skeletal Muscle Atrophy by Regulating Oxidative Stress and Lipid Metabolism in Rats
- 주제(키워드) oxidative stress , muscle atrophy , lipid metabolism , camphene , sarcopenia
- 주제(기타) Nutrition & Dietetics
- 설명문(일반) [Baek, Suji; Lee, Kang Pa] UMUST R&D Corp, Res & Dev Ctr, Seoul 05029, South Korea; [Kim, Jisu] Konkuk Univ, Phys Act & Performance Inst, Seoul 05029, South Korea; [Moon, Byung Seok; Park, Sun Mi; Jung, Da Eun; Kang, Seo Young; Kim, Hye Ok; Yoon, Hai Jeon; Kim, Bom Sahn] Ewha Womans Univ, Coll Med, Dept Nucl Med, Seoul 07804, South Korea; [Lee, Sang Ju; Oh, Seung Jun] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Nucl Med, Seoul 05505, South Korea; [Kwon, Seung Hae; Nam, Myung Hee] Korean Basic Sci Inst, Seoul Ctr, Seoul 02841, South Korea
- 등재 SCIE, SCOPUS
- OA유형 Green Published, gold
- 발행기관 MDPI
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000175169
- 본문언어 영어
- Published As http://dx.doi.org/10.3390/nu12123731
- PubMed https://pubmed.ncbi.nlm.nih.gov/33287349
초록/요약
Sarcopenia- or cachexia-related muscle atrophy is due to imbalanced energy metabolism and oxidative stress-induced muscle dysfunction. Monoterpenes play biological and pharmacological reactive oxygen species (ROS) scavenging roles. Hence, we explored the effects of camphene, a bicyclic monoterpene, on skeletal muscle atrophy in vitro and in vivo. We treated L6 myoblast cells with camphene and then examined the ROS-related oxidative stress using Mito Tracker(TM) Red FM and anti-8-oxoguanine antibody staining. To investigate lipid metabolism, we performed real-time polymerase chain reactions, holotomographic microscopy, and respiratory gas analysis. Rat muscle atrophy in in vivo models was observed using F-18-fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography and immunocytochemistry. Camphene reversed the aberrant cell size and muscle morphology of L6 myoblasts under starvation and in in vivo models. Camphene also attenuated E3 ubiquitin ligase muscle RING-finger protein-1, mitochondrial fission, and 8-oxoguanine nuclear expression in starved myotubes and hydrogen peroxide (H2O2)-treated cells. Moreover, camphene significantly regulated lipid metabolism in H2O2-treated cells and in vivo models. These findings suggest that camphene may potentially affect skeletal muscle atrophy by regulating oxidative stress and lipid metabolism.
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