Acremonidin E produced by Penicillium sp. SNF123, a fungal endophyte of Panax ginseng, has antimelanogenic activities
- 주제(키워드) Acremonidin E , Endophytic fungus , Melanogenesis , Panax ginseng , Penicillium sp. SNF12
- 주제(기타) Plant Sciences
- 주제(기타) Chemistry, Medicinal
- 주제(기타) Integrative & Complementary Medicine
- 설명문(일반) [Kim, Kyuri; Lim, Kyung-Min] Ewha Womans Univ, Coll Pharm, Seoul 03760, South Korea; [Jeong, Hae-In; Nam, Sang-Jip] Ewha Womans Univ, Dept Chem & Nanosci, Global Top 5 Program, Seoul 03760, South Korea; [Yang, Inho] Korea Maritime & Ocean Univ, Dept Convergence Study Ocean Sci & Technol, Busan, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- OA유형 Green Published, gold
- 발행기관 KOREAN SOC GINSENG
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000175390
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.jgr.2019.11.007
- PubMed https://pubmed.ncbi.nlm.nih.gov/33437161
초록/요약
Background: Ginseng extracts and ginseng-fermented products are widely used as functional cosmetic ingredients for their whitening and antiwrinkle effects. Recently, increasing attention has been given to bioactive metabolites isolated from endophytic fungi. However, little is known about the bioactive metabolites of the fungi associated with Panax ginseng Meyer. Methods: An endophytic fungus, Penicillium sp. SNF123 was isolated from the root of P. ginseng, from which acremonidin E was purified. Acremonidin E was tested on melanin synthesis in the murine melanoma cell line B16F10, in the human melanoma cell line MNT-1, and in a pigmented 3D-human skin model, Melanoderm. Results: Acremonidin E reduced melanogenesis in alpha-melanocyte-stimulating hormone (alpha-MSH)-stimulated B16F10 cells with minimal cytotoxicity. qRT-PCR analysis demonstrated that acremonidin E downregulated melanogenic genes, including tyrosinase and tyrosinase-related protein 1 (TRP-1), while their enzymatic activities were unaffected. The antimelanogenic effects of acremonidin E were further confirmed in MNT-1 and a pigmented 3D human epidermal skin model, Melanoderm. Immunohistological examination of the Melanoderm further confirmed the regression of both melanin synthesis and melanocyte activation in the treated tissue. Conclusion: This study demonstrates that acremonidin E, a bioactive metabolite derived from a fungal endophyte of P. ginseng, can inhibit melanin synthesis by downregulating tyrosinase, illuminating the potential utility of microorganisms associated with P. ginseng for cosmetic ingredients. (C) 2019 The Korean Society of Ginseng, Published by Elsevier Korea LLC.
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