Salmonella Vaccine Vector System for Foot-and-Mouth Disease Virus and Evaluation of Its Efficacy with Virus-Like Particles
- 주제(키워드) foot-and-mouth disease , VP1 , live attenuated Salmonella vector , mucosal immunity , virus-like particle , radiation mutation
- 주제(기타) Immunology
- 주제(기타) Medicine, Research & Experimental
- 설명문(일반) [Zhi, Yong; Ji, Hyun Jung; Byun, Eui-Baek; Seo, Ho Seong] Korea Atom Energy Res Inst, Res Div Radiat Sci, Jeongeup 56212, South Korea; [Zhi, Yong; Seo, Ho Seong] Univ Sci & Technol, Dept Radiat Sci, Daejeon 34113, South Korea; [Ji, Hyun Jung] Seoul Natl Univ, Sch Dent, DRI, Dept Oral Microbiol & Immunol, Seoul 08826, South Korea; [Ji, Hyun Jung] Seoul Natl Univ, Sch Dent, BK21 Plus Program, Seoul 08826, South Korea; [Guo, Huichen] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Natl Foot & Mouth Dis Reference Lab, State Key Lab Vet Etiol Biol, Lanzhou 730000, Peoples R China; [Lim, Jae Hyang] Ewha Womans Univ, Coll Med, Dept Microbiol, Seoul 07804, South Korea; [Lim, Jae Hyang] Ewha Womans Univ, Med Ctr, Ewha Educ & Res Ctr Infect, Seoul 07985, South Korea; [Kim, Woo Sik] Korea Res Inst Biosci & Biotechnol, Funct Biomat Res Ctr, Jeongeup 56212, South Korea
- 등재 SCIE, SCOPUS
- OA유형 gold, Green Published
- 발행기관 MDPI
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000175425
- 본문언어 영어
- Published As http://dx.doi.org/10.3390/vaccines9010022
- PubMed https://pubmed.ncbi.nlm.nih.gov/33466461
초록/요약
Foot-and-mouth disease virus (FMDV) causes a highly contagious and devastating disease in livestock animals and has a great potential to cause severe economic loss worldwide. The major antigen of FMDV capsid protein, VP1, contains the major B-cell epitope responsible for effectively eliciting protective humoral immunity. In this study, irradiated Salmonella Typhimurium (KST0666) were used as transgenic vectors containing stress-inducible plasmid pRECN-VP1 to deliver the VP1 protein from FMDV-type A/WH/CHA/09. Mice were orally inoculated with ATOMASal-L3 harboring pRECN-VP1, and FMDV virus-like particles, where (VLPFMDV)-specific humoral, mucosal, and cellular immune responses were evaluated. Mice vaccinated with attenuated Salmonella (KST0666) expressing VP1 (named KST0669) showed high levels of VLP-specific IgA in feces and IgG in serum, with high FMDV neutralization titer. Moreover, KST0669-vaccinated mice showed increased population of IFN-gamma (type 1 T helper cells; Th1 cells)-, IL-5 (Th2 cells)-, and IL-17A (Th17 cells)-expressing CD4(+) as well as activated CD8(+) T cells (IFN-gamma(+)CD8(+) cells), detected by stimulating VLPFMDV. All data indicate that our Salmonella vector system successfully delivered FMDV VP1 to immune cells and that the humoral and cellular efficacy of the vaccine can be easily evaluated using VLPFMDV in a Biosafety Level I (BSL1) laboratory.
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