Extract of high hydrostatic pressure-treated danshen (Salvia miltiorrhiza) ameliorates atherosclerosis via autophagy induction
- 주제(키워드) Atherosclerosis , Autophagy , Cryptotanshinone , High hydrostatic pressure , Salvia miltiorrhiza
- 주제(기타) Biochemistry & Molecular Biology
- 설명문(일반) [Ko, Minjeong; Kwon, Ho Jeong] Yonsei Univ, Coll Life Sci & Biotechnol, Dept Biotechnol, Chem Genom Global Res Lab, Seoul 03722, South Korea; [Oh, Goo Taeg] Ewha Womans Univ, Dept Life Sci, Seoul 03762, South Korea; [Park, Jiyong] Yonsei Univ, Coll Life Sci & Biotechnol, Dept Biotechnol, Seoul 03722, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- OA유형 gold, Green Published
- 발행기관 KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000175455
- 본문언어 영어
- Published As http://dx.doi.org/10.5483/BMBRep.2020.53.12.184
- PubMed https://pubmed.ncbi.nlm.nih.gov/33172543
초록/요약
Danshen (Salvia miltiorrhiza) is a traditional medicinal plant widely used in Asian countries for its pharmacological activities (e.g., amelioration of cardiovascular diseases). In this study, we investigated the anti-atherosclerotic activity of raw danshen root extract prepared using high hydrostatic pressure (HHP) at 550 MPa for 5 min and hot water extraction. This method was useful for elimination of bacteria from cultured danshen plants and for better extraction yield of active principles. The HHP-treated danshen extract (HDE) inhibited proliferation of human umbilical vein endothelial cells (HUVECs) and induced autophagy that was assessed by LC3 conversion and p62 degradation. HDE suppressed foam cell formation in oxLDL-induced RAW264.7 macrophages; lysosomal activity simultaneously increased, measured by acridine orange staining. HDE also reduced atherosclerotic plaque development in vivo in apolipoprotein E knock-out (ApoE(-/-)) mice fed a high cholesterol diet. Taken together, these results indicated that HDE exhibited anti-atherosclerotic activity via autophagy induction.
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