Efficient bioconversion of sugarcane bagasse into polyhydroxybutyrate (PHB) by Lysinibacillus sp. and its characterization
- 주제(키워드) Corn steep liquor , Lysinibacillus sp. RGS , Peracetic acid pretreatment , Poly(3-hydroxybutyrate) , Saccharification yield , Sugarcane bagasse
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier Ltd
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000175592
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.biortech.2021.124673
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
In this study, Lysinibacillus sp. RGS was evaluated to synthesize polyhydroxybutyrate (PHB) from a broad range of pure carbon sources and residual sugars of chemically pretreated sugarcane bagasse (SCB) hydrolysates. Effects of supplementation of nutrients and various experimental variables to enhance PHB accumulation were investigated. Results of optimized parameters were identified as 48 h, 37 °C, pH 7; inoculums concentration (2.5% v/v) and shaking condition (100 rpm). Growth kinetics and bioprocess parameters of Lysinibacillus sp. using SCB hydrolysates with corn steep liquor (2%) accounted for the maximum cell growth (8.65 g/L) and PHA accumulation (61.5%) with PHB titer of (5.31 g/L) under optimal conditions. The produced biopolymer was studied by Fourier Transform Infrared (FTIR) spectroscopy and the results revealed the obtained to be PHB. Thus Lysinibaciluus sp. exhibits high potential in industrial scale manufacture of PHB using SCB as an inexpensive substrate. © 2021 Elsevier Ltd
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