Tamoxifen-inducible cardiac-specific Cre transgenic mouse using VIPR2 intron
- 주제(키워드) VIPR2 intron , ERT2CreERT2 , Heart , Tamoxifen-inducible Cre transgenic mouse
- 주제(기타) 수의학
- 설명문(URI) https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART002631849
- 등재 KCI등재
- 발행기관 한국실험동물학회
- 발행년도 2020
- URI http://www.dcollection.net/handler/ewha/000000181301
- 본문언어 영어
- Published As http://dx.doi.org/10.1186/s42826-020-00065-x
초록/요약
Genetically engineered mouse models through gene deletion are useful tools for analyzing gene function. To delete a gene in a certain tissue temporally, tissue-specific and tamoxifen-inducible Cre transgenic mice are generally used. Here, we generated transgenic mouse with cardiac-specific expression of Cre recombinase fused to a mutant estrogen ligand-binding domain (ERT2) on both N-terminal and C-terminal under the regulatory region of human vasoactive intestinal peptide receptor 2 ( VIPR2 ) intron and Hsp68 promoter ( VIPR2-ERT2CreERT2 ). In VIPR2-ERT2CreERT2 transgenic mice, mRNA for Cre gene was highly expressed in the heart. To further reveal heart-specific Cre expression, VIPR2-ERT2CreERT2 mice mated with ROSA26-lacZ reporter mice were examined by X-gal staining. Results of X-gal staining revealed that Cre-dependent recombination occurred only in the heart after treatment with tamoxifen. Taken together, these results demonstrate that VIPR2-ERT2CreERT2 transgenic mouse is a useful model to unveil a specific gene function in the heart.
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