Fermentative High-Level Production of 5-Hydroxyvaleric Acid by Metabolically Engineered Corynebacterium glutamicum
- 주제(키워드) 5-hydroxyvaleric acid , Corynebacterium glutamicum , L-lysine , glutaric acid , 5-aminovaleric acid
- 주제(기타) Chemistry, Multidisciplinary
- 주제(기타) Green & Sustainable Science & Technology
- 주제(기타) Engineering, Chemical
- 설명문(일반) [Sohn, Yu Jung; Baritugo, Kei-Anne; Son, Jina; Park, Si Jae] Ewha Womans Univ, Syst Hlth & Engn Major Grad Sch, Div Chem Engn & Mat Sci, BK21 Plus Program, Seoul 03760, South Korea; [Kang, Minsoo; Kang, Kyoung Hee; Ryu, Mi-Hee; Sohn, Mingi; Jung, Ye Jean; Kim, Hee Taek] Korea Res Inst Chem Technol, Ctr Biobased Chem, Div Specialty & Biobased Chem Technol, Daejeon 34602, South Korea; [Kang, Minsoo; Jung, Ye Jean; Park, Kyungmoon] Hongik Univ, Dept Biol & Chem Engn, Sejong Si 30016, South Korea; [Lee, Siseon; Joo, Jeong Chan] Catholic Univ Korea, Dept Biotechnol, Bucheon Si 14662, Gyeonggi Do, South Korea
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2021
- URI http://www.dcollection.net/handler/ewha/000000181390
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acssuschemeng.0c08118
초록/요약
We report metabolic engineering of Corynebacterium glutanucum C. glutamicum) for high-level production of 5-hydroxyvaleric acid (S-HV), an important C5 platform chemical covering a wide range of industrial applications, using glucose as a sole carbon source. To derive 5-HV, an artificial S-HV biosynthesis pathway, composed of the first three reaction steps of an L-lysine catabolic pathway via 5-aminovaleramide along with a subsequent intracellular reduction step, was constructed: L-lysine was converted to glutarate semialdehyde through an L-lysine catabolic pathway encoded by Pseudomonas putida davTBA genes, and glutarate semialdehyde was further reduced to 5-HV by a suitable aldehyde reductase. Various aldehyde reductases including CpnD from Clostridium aminovalericum, Gbd from Ralstonia eutropha, ButA from C. glutamicum, and YihU, YahK, and YqhD from Escherichia coli were examined for efficient 5-HV production through the flask and batch cultivations, and YahK was determined to be the most appropriate aldehyde reductase. Further modification to enhance 5-HV production was investigated by deletion of an endogenous gabD gene responsible for the oxidation of glutarate semialdehyde into glutaric acid in order to suppress glutaric acid by-production. Finally, 52.1 g/L S-HV with the yield of 0.33 g/g glucose was achieved by fed-batch fermentation of the engineered C. glutamicum with overexpression of davTBA genes and the yahK gene along with gabD deletion in the chromosome.
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