Increased Antibiotic Resistance of Methicillin-Resistant Staphylococcus aureus USA300 Delta psm Mutants and a Complementation Study of Delta psm Mutants Using Synthetic Phenol-Soluble Modulins
- 주제(키워드) MRSA , phenol-soluble modulins , beta-lactam antibiotic , biofilm , fatty acid , persister cell
- 주제(기타) Biotechnology & Applied Microbiology
- 주제(기타) Microbiology
- 설명문(일반) [Song, Hun-Suk; Bhatia, Shashi Kant; Choi, Tae-Rim; Gurav, Ranjit; Kim, Hyun Joong; Lee, Sun Mi; Park, Sol Lee; Lee, Hye Soo; Yang, Yung-Hun] Konkuk Univ, Coll Engn, Dept Biol Engn, Seoul 05029, South Korea; [Bhatia, Shashi Kant; Yang, Yung-Hun] Konkuk Univ, Inst Ubiquitous Informat Technol & Applicat CBRU, Seoul 14662, South Korea; [Joo, Hwang-Soo] Duksung Womens Univ, Coll Engn, Dept Biotechnol, Seoul 01369, South Korea; [Kim, Wooseong] Ewha Womans Univ, Coll Pharm, Seoul 03760, South Korea; [Kim, Wooseong] Ewha Womans Univ, Grad Sch Pharmaceut Sci, Seoul 03760, South Korea; [Seo, Seung-Oh] Catholic Univ Korea, Dept Food Sci & Nutr, Bucheon 14662, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- 발행기관 KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- 발행년도 2021
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000181416
- 본문언어 영어
- Published As http://dx.doi.org/10.4014/jmb.2007.07034
초록/요약
Phenol-soluble modulins (PSMs) are responsible for regulating biofilm formation, persister cell formation, pmtR expression, host cell lysis, and anti-bacterial effects. To determine the effect of psm deletion on methicillin-resistant Staphylococcus aureus, we investigated psm deletion mutants including Delta psm alpha, Delta psm beta, and Delta psm alpha beta. These mutants exhibited increased beta-lactam antibiotic resistance to ampicillin and oxacillin that was shown to be caused by increased N-acetylmannosamine kinase (nanK) mRNA expression, which regulates persister cell formation, leading to changes in the pattern of phospholipid fatty acids resulting in increased anteiso-C-15:0, and increased membrane hydrophobicity with the deletion of PSMs. When synthetic PSMs were applied to Delta psm alpha and Delta psm beta mutants, treatment of Delta psm alpha with PSM alpha 1-4 and Delta psm beta with PSM beta 1-2 restored the sensitivity to oxacillin and slightly reduced the biofilm formation. Addition of a single fragment showed that alpha 1, alpha 2, alpha 3, and beta 2 had an inhibiting effect on biofilms in Delta psm alpha; however, beta 1 showed an enhancing effect on biofilms in Delta psm beta. This study demonstrates a possible reason for the increased antibiotic resistance in psm mutants and the effect of PSMs on biofilm formation.
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